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Chinese Agricultural Science Bulletin ›› 2011, Vol. 27 ›› Issue (4): 12-16.

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Study on the Cause of Leaf Etiolation Virus of Populus tomentosa ‘Huaye’

  

  • Received:2010-09-14 Revised:2010-11-22 Online:2011-02-10 Published:2011-02-10

Abstract:

In order to search factors related to the forming of mosaic leaf of Populus tomentosa ‘Huaye’which was used as the research material, Nicotiana tabacum, Glycine max and Populus beijingensis were used as show plants. Inoculability, ELISA, distill RNA test and RT-PCR method were used to detect yellow mosaic virus of Populus tomentosa ‘Huaye’ .The results showed that: (1) The plant of Nicotiana tabacum, Glycine max and Populus beijingensis didn’t show wrinkle and losing green after rubbing or injecting inoculability by using the percolate of Populus tomentosa ‘Huaye’. (2) ELISA didn’t show masculine. (3) The electrophoresis fig of Populus tomentosa ‘Huaye’ didn’t show bright strip. (4) There were no amplified products by RT-PCR. Plant Virus didn’t cause yellow mosaic of Populus tomentosa ‘Huaye’.