Abstract:

It was reported that aberrant mitochondrial gene orf25 might cause tobacco cytoplasmic male sterility (CMS). To understand the mechanism of CMS, the differences of orf25 gene between CMS line ‘Gexin 3’ and its maintainer line were analyzed by bioinformatics at different structure levels of coding protein. The results showed that the terminal codon appeared early because of the base change in the orf25 gene, which led to the lack of the structural domain in coding protein. It might be the key factor that disturbed the function of F(0)F(1)-ATP synthetase in mitochondrion, and become one of the fundamental causes which led to CMS of tobacco.