Abstract:

To study the action mechanism of PP333 on nurturing in vitro preserved strong seedling of potato test-tube plantlets and on delaying its aging, potato test-tube plantlets were used as explants, different concentrations of PP333 were added into MS culture media to determine the activity of antioxidase (CAT, POD, SOD etc.) and the dynamic physiological indices of MDA and soluble protein content. The results showed that samples treated with 0.01, 0.05 and 0.25 mg/L PP333 had an increased antioxidase (CAT, POD, SOD etc.) activity, kept a relatively low content level of MDA and their decreasing of soluble protein content was delayed. The samples treated with 1.25 mg/L PP333 had a low SOD activity and their soluble protein content was decreased significantly, though they kept a relatively high CAT and POD activities. In conclusion, low concentration of PP333 can help nurturing strong seedlings and delaying the aging of test-tube plantlets by keeping a low MDA concentration and a high antioxidase and protein level while high concentration (1.25 mg/L) of PP333 showed detrimental effects by lowering the content of protein and the activity of SOD.