Abstract:

For further study of RSV (rice stripe virus) on transmission rate of between planthoppers and rice plants, in the present study we used the susceptible varieties of rice stripe virus, ‘Wuyujing 7’, and the planthoppers were fed with the healthy rice plants for 1.5 h, 3.0 h, 6.0 h, 12.0 h, 1.0 day, 2.0 days and 4.0 days respectively, and RT-PCR was employed to detect the virus in rice at the different times after feeding. The results showed that the vector planthoppers could transmit the virus successfully to rice plants at 3.0 h after feeding, and the probability that rice plants were infected increased as the feeding time extended. We also used a dot immunobinding assay (DIBA) to detect acquisition of RSV in planthoppers after the virus-free planthoppers fed on RSV-infected rice plants for 0.25 h, 0.5 h, 1.0 h, 2.0 h, 4.0 h and 8.0 h respectively. The data revealed that the planthoppers could acquire the virus 0.5 h after feeding, and the long feeding time increased the probability of the planthoppers infection. This was the first at the molecular level that the RSV content of the rice plants fed on the vector planthoppers was detected, and at the same time, the rate that planthoppers obtained RSV from RSV-infected rice plants was combined with, which provided a new way for rice stripe disease forecasting.