Abstract:

In order to clarify the sensitivity of mannose in the regeneration of different genotypes sugarcane, and it will provide the techniques for genetic engineering breeding of sugarcane. Research on the regeneration of sugarcane varieties of ‘ROC22’, ‘GT94-119’ and ‘GT96-44’ in media with mannose stress was carried out in this work. The selection system for three sugarcane varieties in additional mannose was established. The proportion of the additional mannose was variant in different regeneration stages, 60% for calli subculture and multiplication, 60%~70% for differentiation and 30% for rooting of plantlets. According to the investigated results by statistical analysis, the affect of mannose on three genotypes of sugarcane was variant in different regeneration stages. There was no significant difference in the subculture stage, but the significant differences were found in differentiation and rooting stages.