Abstract:

A Trichoderma isolated from soil (TlCC) was identified as Trichoderma longibrachiatum on the basis of their morphology and DNA sequence analysis of ITS rDNA. The biological characteristics of TlCC were screened and investigated. Though it could grow on PSA, PMA, NA, Czapek and basic cultrure medium, PDA was the most favorable medium for the hypha growth. It could grow and produce spore from 15℃ to 40℃, but the optimal temperature for the growth was 35℃, and for the sporulation was from 25℃ to 35℃. For the basic culture medium, fructose was optimal carbon source for hypha growth, fructose and tragantine were propitious to spore production. The best nitrogen source for hypha growth and spore production was glutamate. The pH for hyphae growth and spore production ranged between 2 and 10, the optimal pH for hyphae growth was 5, from 3 to 7 were propitious to spore production.