Abstract: According to the data of the statistical yearbook of Anhui province in 2003 ,this paper calculate and analyzes the ecological footprint of Anhui province in 2003 .The result indicates that the ecological deficit per capita of Anhui province was 0.89 hm2 in 2003 ,which reflects that the human load has already stayed beyond the regional bio-capacity, and regional natural ecosystem is under great pressure of human activities.