Abstract:

In order to determine DNA content of nuclear DNA of Ryegrass cultivars, the chromosome was measured by Flow Cytometry. At the same time, it determinated the DNA content and pliody of Ryegrass by the traditional method of chromosome. The result showed that, with the increase of ploidy level, the relative content of nuclear DNA presented doubling. The two detection methods were consistent. However, the method which used nuclear DNA content by Flow Cytometry was simple, rapid and high precision.