Abstract:

More and more researchers focus on genotyping errors because of their effect on the biological conclusions of a study. Using the fluorescent microsatellite genotyping of wild and cultivated eggplants (Solanum melongena L.) as example, error rates had been limited from these two processes that are PCR (polymerase chain reaction) amplification and identification of the fluorescence peak in this study. The rapid and simple protocol was created for optimizing PCR amplification conditions. And some strategies were proposed to discern and assess errors from the fluorescence data. Our study provides some reference for experiment operation and data handling of microsatellite markers.