IMAGE/TABLE DETAILS

• SSR Polymorphism in Sugar Beet: Detection by Polyacrylamide Gel Electrophoresis and Capillary Electrophoresis FENG Wanqi, ZHANG Fushun, LIU Naixin Chinese Agricultural Science Bulletin    2022, 38 (36): 34-41.   DOI: 10.11924/j.issn.1000-6850.casb2022-0412 Abstract   （219） HTML （4） PDF（pc） （1707KB）（140）   In order to explore the applicability of capillary electrophoresis and polyacrylamide electrophoresis and compare their advantages and disadvantages, 8 sugar beet cultivation resources were used as materials, 8 SSR loci were selected. The polymorphism of each locus was detected by polyacrylamide gel electrophoresis silver staining and capillary electrophoresis. And then the results of the two methods were compared. Capillary electrophoresis fluorescence labeling method could accurately obtain the molecular size of DNA fragments. PAGE electrophoresis could only estimate the approximate molecular weight with the naked eye compared with Marker, but could not obtain the accurate molecular weight. The matching ratios of the two detection methods with the 8 pairs of primers were 75%, 87.5%, 50%, 75%, 25%, 100%, 37.5% and 50%, respectively. According to the results, there were great differences in data integration between the two detection platforms. The detection results were directly related to the factors like whether the primers were single copy, primer polymorphism and stability. The sequencing test results showed that when there were fewer samples, using PAGE electrophoresis to detect the polymorphism of SSR loci could not only save cost and time, but also ensure the accuracy of the test results. 引物 分型结果 个体编号 红饲料甜菜 Regina红 96001/2红 Ruby queen BETA176 BTS8840 HI0936 VF3019 匹配比率/% BvCA2 目标峰对应分子量 198 198/194 198 194 194 194 194/198 198 75 毛细管电泳分型结果 1 2 1 1 1 1 2 1 PAGE分型结果 1 1 1 1 1 1 1 1 Bvv23 目标峰对应分子量 96/108 96/108 96/108 96 108 96/108 96 96 87.5 毛细管电泳分型结果 2 2 2 1 1 2 1 1 PAGE分型结果 2 2 2 2 1 2 1 1 Bvv45 目标峰对应分子量 — 212 140 212/231 212/231 231/212 212/231 212 50 毛细管电泳分型结果 — 1 1 2 2 2 2 1 PAGE分型结果 1 2 1 2 2 3 2 2 GTT1 目标峰对应分子量 123 123 117 — 117/123 117/123 123 117/123 75 毛细管电泳分型结果 1 1 1 — 2 2 1 2 PAGE分型结果 1 1 1 1 2 2 2 2 SB06 目标峰对应分子量 130 114.2 117.5 — 131 131 — 130 25 毛细管电泳分型结果 1 1 1 — 1 1 — 1 PAGE分型结果 2 2 2 1 1 2 1 1 SB13 目标峰对应分子量 133/127 133/127 127/133 127/133 133 127/133 133/127 127/133 100 毛细管电泳分型结果 2 2 2 2 1 2 2 2 PAGE分型结果 2 2 2 2 1 2 2 2 SB15 目标峰对应分子量 140 143/152 147/168 138/143/147/52 168/147/143 147/168 147/168 143/168 37.5 毛细管电泳分型结果 1 2 2 4 3 2 2 2 PAGE分型结果 1 2 3 2 3 1 1 1 SSD6 目标峰对应分子量 154/182 168/176 182 176/182 176/168 154/168 168/176/182 176/168/182 50 毛细管电泳分型结果 2 2 1 2 2 2 3 3 PAGE分型结果 3 2 1 2 3 3 3 2 表3 8个甜菜品种在8对SSR引物上的毛细管电泳和PAGE结果比较 Other Images/Table from this Article 表1 甜菜品种信息表 表2 SSR引物 图1 (A)：引物为BvCA2、Bvv23、Bvv45、GTT1、SB06、SB13；(B)：引物为SB15、SSD6 (a)：140 bp；(b)：212 bp、231 bp；(c)：212 bp (a)：147 bp、154 bp、168 bp；(b):143 bp；(c)：143 bp、147 bp、168 bp；(d)：143 bp、147 bp；(e)：143 bp、147 bp、152 bp、154 bp；(f)：138 bp、143 bp、147 bp、153 bp