IMAGE/TABLE DETAILS

• Isolation and Identification of Aeromonas Veroni from Diseased Acrossocheilius Hemispinus ZHANG Jingjing Chinese Agricultural Science Bulletin    2023, 39 (20): 138-146.   DOI: 10.11924/j.issn.1000-6850.casb2022-0781 Abstract   （135） HTML （3） PDF（pc） （1802KB）（18）   The bacterial isolation, identification and drug sensitivity of diseased Acrossocheilius hemispinus with skin ulceration, surface hemorrhage, swollen and bleeding liver were studied. The bacterial strain was isolated and purified from liver tissue of the diseased fish and injected intramuscularly into healthy Acrossocheilius hemispinus. Full-length sequences of 16S rDNA and gyrB gene were obtained by PCR amplification of pathogenic bacteria, while physiological and biochemical identification, virulent genes detection, and drug sensitivity study were also conducted. The isolated strain was numbered as AS0829L1, and the artificial infection experiments showed that pathogenic groups of 5×1010, 5×109, 5×108 CFU/mL could cause 100%, 100%and 28.6% mortality, respectively. The BLAST result indicated the sequences of 16S rDNA and gyrB were homologous to the corresponding sequence of Aeromonas veronii in Genbank, with homology as high as 99.93% and 99.34%, respectively. Comprehensive molecular biology, physiological and biochemical characteristics, virulence gene detection results demonstrated the separate strain was Aeromonas veronii bv.sobria. The drug sensitivity test showed the isolated strain was highly sensitive to 20 antibacterial drugs including cefuroxime, cefoperazone, gentamicin, neomycin, enrofloxacin and ofloxacin, moderate sensitive to 5 antibacterial drugs including carboxybenzillin, streptomycin, flufenicol, and resistant to 12 antibacterial drugs including penicillin, norfloxacin, erythromycin. In this study, the pathogen of the diseased Acrossocheilius hemispinus was identified and the drug sensitivity test of the isolated strain was carried out, which provided a research basis for the prevention and control of diseases in aquaculture production. 测定项目 AS0829L1 测定项目 AS0829L1 测定项目 AS0829L1 测定项目 AS0829L1 丙氨酸-苯丙氨酸-脯氨酸芳胺酶 - β-丙氨酸芳胺酶pNA - D-纤维二糖 - 5-酮基-D-葡萄糖酸盐 - 谷氨酸-甘氨酸-精氨酸芳胺酶 + 甘氨酸芳胺酶 - D-塔格糖 - 柠檬酸盐(钠) - L-脯氨酸芳胺酶 + α-葡糖苷酶 + D-葡萄糖 + 丙二酸盐 - L-吡咯烷酮芳胺酶 - β-木糖苷酶 - D-甘露糖 + L-苹果酸盐同化 + 鸟氨酸脱羧酶 - 磷酸酶 - 海藻糖 + L-乳酸盐同化 - α-半乳糖苷酶 + 尿素酶 - 古老糖 - L-组氨酸同化 - β-葡萄糖醛酸酶 - β-半乳糖苷酶 + D-麦芽糖 + L-乳酸盐碱化 - β-N-乙酰氨基葡萄糖苷酶 + β-葡糖苷酶 - 发酵/葡萄糖 + 0/129耐(comp.vibrio) + 谷氨酰芳胺酶pNA + 脂酶 - 侧金盏花醇 - 香豆酸 + 赖氨酸脱羧酶 - 酪氨酸芳胺酶 + D-山梨醇 - ELLMAN - γ-谷氨酰转移酶 + 蔗糖 + D-甘露醇 + H2S产生 - 表3 分离菌株AS0829L1生理生化鉴定结果 Other Images/Table from this Article 表1 毒力基因引物序列及扩增片段大小 图1 图2 表2 分离菌株AS0829L1回归实验结果 图3 A：体表发红、溃烂、鳞片脱落、烂尾（箭头）；B：肝脏发黄发白、脾脏发黑肿大（箭头） 图4 图5 M-marker；1-act；2-ast；3-aer A；4-alt；5-hly A，6-tap A 表4 分离菌株AS0829L1药敏试验结果