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  Elsinoe batatas Causing Sweetpotato Scab: Biological Characteristics and Conidia Production ZHANG Xinxin, HUANG Yong, CHEN Jingyi, ZOU Hongda, LUO Zhongxia, WANG Zhangying, HUANG Lifei Chinese Agricultural Science Bulletin    2024, 40 (3): 112-119.   DOI: 10.11924/j.issn.1000-6850.casb2023-0081 Abstract   （167） HTML （3） PDF（pc） （2093KB）（18）   The biological characteristics of the pathogen of sweetpotato scab (Elsinoe batatas) were studied and the methods of producing a large number of conidia in a short time by E. batatas on artificial medium were screened to provide the basis for resistance breeding of sweetpotato scab. Taking the E. batatas isolate CRI-CJ2 as research object, the mycelial growth rate method was utilized to measure the colonial diameter under different medium, pH values and illumination conditions. The concave slide pendent drop method was used to study the conidial germination rate under different temperature, pH values and illumination conditions, and the effects of the Fries liquid culture method, shaking method and mycelium coating method for inducing conidia production of E. batatas were compared. The results showed that the optimal medium for mycelial growth was PDA, the optimum pH value was 6-7, and the lethal temperature was 48℃ for 10 min. No significant differences were found in the mycelial growth between different illuminations, but the colour of colony turned bright red under continuous illumination condition. The optimum pH value for conidial germination was 5, the best temperature was 25℃, and the conidia could not germinate above 48℃ for 10 min. Illumination showed little effect on conidial germination. The conidial quantity of E. batatas was 7.09×107 conidia/mL after 12 days of incubation by the mycelium coating method and the conidial germination rate was 24% after incubation for 24 hours, and the quantity and viability were higher than that by the shaking method. No conidia or sporogenous structure were observed by the Fries liquid culture method. Therefore, selecting appropriate medium and pH value could promote mycelial growth, and the mycelium coating method had the advantages of rapid sporulation and high conidial quantity and was a suitable method for E. batatas conidia induction. 图6 Other Images/Table from this Article 表1 不同培养基对甘薯疮痂病菌菌落生长的影响 a：PDA，b：SpSA，c：SpSDA 表2 不同光照条件对甘薯疮痂病菌菌落生长的影响 图2 a：连续光照；b：12 h光/暗交替；c：连续黑暗 表3 不同pH对甘薯疮痂病菌菌落生长的影响 a：pH 4；b：pH 5；c：pH 6；d：pH 7；e：pH 8；f：pH 9；g：pH 10 图4 图5 图7 a，b：甘薯疮痂病菌利用Fries液体培养法的培养性状及产生的厚垣孢子形态；c，d：甘薯疮痂病菌利用摇菌法的培养性状及产生的分生孢子形态；e，f：甘薯疮痂病菌利用菌丝涂布法的培养性状及产生的分生孢子形态 图8