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• New Wheat Variety ‘Kexing 3302’: Detection and Analysis of High Quality and Yield Related Genes LYU Chunlei, HAO Shuang, BAI Xintong, WU Enzhao, YANG Meng, CAO Pengbo, WANG Daowen, ZHANG Kunpu Chinese Agricultural Science Bulletin    2025, 41 (25): 1-8.   DOI: 10.11924/j.issn.1000-6850.casb2025-0060 Abstract   （122） HTML （8） PDF（pc） （1620KB）（113）   The aim is to understand the molecular genetic basis of high quality and high yield of new wheat variety ‘Kexing 3302’. ‘Kexing 3302’ was used as the test material，and molecular markers related to quality, dwarf, growth and development, and disease resistance were used for detection. The results showed that ‘Kexing 3302’contained high molecular weight glutenin subunit combination Ax1G330E, Bx7+By8, Dx5+Dy10, low molecular weight glutenin Glu-A1a, non-1BL /1RS translocation line, which were the genetic basis of high quality; four dwarf genes Rht1, Rht2, Rht8 and Rht24, which were the genetic basis of lodging resistance and high and stable yield of the dwarf; three recessive vernalization genes Vrn-A1b, Vrn-B1b and Vrn-D1b as well as photopericyclic gene insensitivity gene Ppd-D1a, which were the genetic basis of cold resistance and wide adaptability; and QYm.njau-2D locus and anti-stripe rust gene Yr10, which were the genetic basis of high resistance to yellow Mosaic virus and stripe rust disease. The genes of quality, dwarf, growth and disease resistance in ‘Kexing 3302’ were consistent with the phenotype of high quality and yield, disease resistance, laying a foundation for the efficient utilization of the excellent genes of ‘Kexing 3302’ in molecular breeding. 性状 位点 引物或标记名称 引物序列(5’-3’) 片段长度/bp 退火温度/℃ 文献 高分子量麦谷蛋白亚基 Glu-A1 1AX F：GCCCAATAATTTGTAAGTCCA 882/719/594 60 [4] R：ACAACTATTTCGGAACAGAGG Glu-B1 Bx7/17 F：CGCAACAGCCAGGACAATT 630/766/669 58 [5] R：AGAGTTCTATCACTGCCTGGT By8 F：TTAGCGCTAAGTGCCGTCT 527 58 [5] R：TTGTCCTATTTGCTGCCCTT Glu-D1 Xrj2 F：CTCGCTCGACGGTGTTGAA 1085/428 60 [6] R：CCTTTGGCCCAGATAAAGTG 低分子量麦谷蛋白亚基 Glu-A1 Glu-A3a F：GTACGCTTTTGTAGCTTGTGC 596 60 [7] R：TGGTGGTTGTTGTTGTTGCTACA 1BL/1RS Sec-1 bmac0213 F：ATGGATGCAAGACCAAAC 524 50 [4] R：CTATGAGAGGTAGAGCAGCC 矮杆基因 Rht1 NH-BF.2 F：TCTCCTCCCTCCCCACCCCAAC 400 63 [8] R：CATCCCCATGGCCATCTCGAGCTA Rht2 DF F：CGCGCAATTATTGGCCAGAGATAG 254 63 [8] R：CCCCATGGCCATCTCGAGCTGCTA Rht8 DG273 F：CTTGACGAGCTTGGAAATGG 421 55 [9] R：GCAACAAGTGCTTCTGTCGT Rht24 TaGA2ox F：TCGCAACTTTTGCTAGGCAAT 601 58 [10] R：CAGGAGCAATCTACAGCAGAAAG 春化基因 Vrn-A1 Intr1/C F：GCACTCCTAACCCACTAACC 1086 59 [11] Intr1/AB R：TCATCCATCATCAAGGCAAA Vrn-B1 Intr1/B F：CAAGTGGAACGGTTAGGACA 1149 58 [11] R：CAAATGAAAAGGAATGAGAGCA Vrn-D1 Intr1/D F：GTTGTCTGCCTCATCAAATCC 1671 63 [11] Intr1/D-3 R：GGTCACTGGTGGTCTGTGC Intr1/D F：GTTGTCTGCCTCATCAAATCC 997 59 [11] Intr1/D-4 R：AAATGAAAAGGAACGAGAGCG 光周期基因 Ppd-D1a 2D-Ins F：ACGCCTCCCACTACACTG 288 50 [12] 2D-Ins-1 R：GTTGGTTCAAACAGAGAGC Ppd-D1b 2D-Ins F：ACGCCTCCCACTACACTG 414 50 [12] 2D-Ins-2 R：CACTGGTGGTAGCTGAGAT 抗条锈病基因 Yr10 SC200 F：CTGCAGAGTGACATCATACA 230 57 [13] R：TCGAACTAGTAGATGCTGGC 抗黄花叶病毒病基因 Qym.nau-2D mag4059 F：TGACAGCAGGAAAGAGTCCA 250 55 [33] R：AGCTGCCTGGAGAGCAAGAT 表1 分子标记引物 Other Images/Table from this Article 图1 a.Glu-A1位点的分子标记检测；b.Glu-B1位点的Bx7/Bx17基因的分子标记检测；c.Glu-B1位点的By8基因的分子标记检测；d.Glu-D1位点的分子标记检测。M为DNA Maker 2000 M为DNA Maker 2000 M为DNA Maker 2000 图4 a.Rht1基因的分子标记检测；b.Rht2基因的分子标记检测；c.Rht8基因的分子标记检测；d.Rht24基因的分子标记检测。M为DNA Maker 2000 图5 a.Vrn-A1b基因的分子标记检测；b.Vrn-B1b基因的分子标记检测； c.Vrn-D1a基因的分子标记检测；d.Vrn-D1b基因的分子标记检测。M为DNA Maker 2000 M为DNA Maker 2000 M为DNA Maker 2000 M为DNA Maker 2000