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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (17): 183-186.

所属专题： 生物技术； 园艺

• 农学 农业基础科学 • 上一篇    下一篇

豌豆叶片细胞中茉莉酸的胶体金免疫电镜定位

刘艳   

  • 收稿日期:2010-01-11 修回日期:2010-06-22 出版日期:2010-09-05 发布日期:2010-09-05
  • 基金资助:

    H2O2在茉莉酸诱导鲜切豌豆芽苗伤害反应形成信号通道中作用

Subcellular Localization of Jasmonic Acid by Immunogold Electron Microscopy Technique in Pea Leaves

  • Received:2010-01-11 Revised:2010-06-22 Online:2010-09-05 Published:2010-09-05

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摘要：

摘要：利用胶体金免疫电镜定位技术对豌豆（Pisum sativum L.）叶片细胞中茉莉酸进行亚细胞定位。结果显示，在叶肉细胞中，茉莉酸主要分布在叶绿体、细胞壁和细胞质中，细胞核中也有少量金颗粒分布。韧皮部筛管与伴胞分子中观察到有大量的金颗粒标记，木质部细胞中没有观察到金颗粒标记。上述研究结果为阐明茉莉酸生物学功能提供重要细胞学依据。

关键词: 小麦, 小麦, 生长发育, 干旱, 昆明

Abstract:

Abstract: Subcellular localization of jasmonic acid (JA) was performed by means of immunogold electron microscopy technique in pea (Pisum sativum L.) leaves. The result showed that a large number of JA visualized by immunogold particles distributed in the chloroplast , cell wall and cytoplasm of mesophyll cells, a few in the nuclei. In phloem, most of immunogold particles were observed in the sieve element-companion cell (SE-CC) complex, whereas little immunogold particle was found in the xylem. The present results offered a cytological basis for further study on biological function of JA in plant.