欢迎访问《中国农学通报》,

中国农学通报 ›› 2008, Vol. 24 ›› Issue (11): 160-164.doi: 10.11924/j.issn.1000-6850.2008-0790

所属专题: 生物技术 园艺

• 林学 园艺 园林 • 上一篇    下一篇

邻羟基苯甲酸胁迫对不同杉木无性系叶片核酸和蛋白质合成的影响

杨梅,林思祖,曹光球   

  • 收稿日期:2008-09-02 修回日期:2008-09-20 出版日期:2008-11-05 发布日期:2008-11-05

The effects of salicylic acid stress on the leaves nucleic acid and protein synthesis

Yang Mei, Lin Sizu, Cao Guangqiu   

  • Received:2008-09-02 Revised:2008-09-20 Published:2008-11-05 Online:2008-11-05

摘要: 以对邻羟基苯甲酸胁迫具有不同抗性2个杉木无性系(即忍耐型和敏感型)为对象,分析胁迫对叶片遗传物质合成的影响。结果表明:邻羟基苯甲酸胁迫对忍耐型的DNA合成多表现为抑制效应,RNA合成多表现为促进效应,而对敏感型的DNA合成多表现为促进效应,RNA合成表现为抑制效应;在胁迫初期时各胁迫浓度对敏感型的蛋白质含量的促进效应大于忍耐型,但在胁迫中后期,而对敏感型的促进效应呈下降趋势,而对忍耐型的促进效应随胁迫浓度加大而不断增大,蛋白质合成能力不断提高。表明不同抗性杉木无性系基因表达调控模式存在较大差别,随着胁迫强度增大,忍耐型RNA和蛋白质的合成量增加,使更多的蛋白质参与了对此种胁迫的响应,而使其有更好的抗逆性。

关键词: RS, RS, GIS, 绿地系统规划, 空间分析

Abstract: The responses of nucleic acid and protein in leaf were analyzed objecting to two Chinese fir clones with different resistance to salicylic acid stress. The results showed that, in most situations, salicylic acid stress inhabited the DNA synthesis of endurance type clone and promoted its RNA synthesis and the effect characteristics on the sensitivity type was opposite to the endurance type. At the initial period all concentration levels had higher stimulatory effects on the protein content of the sensitivity type than that of endurance type. At the intermediate and later periods, the stimulatory effect on sensitivity type appeared decreasing trend, while the stimulatory effect on endurance type increased continuously with the increasing concentration. Those evidences hinted there were different genetic expression modulations between the two resistance types. The RNA and protein contents of endurance type increased with the increasing stress intensity, which indicated that it aroused more protein to participate in the stress response to enhance its resistance.