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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (24): 20-23.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

零售鲜猪肉中肠球菌的鉴定与毒力基因检测

段志刚,王亚宾,胡 惠,金 钺,崔保安   

  • 收稿日期:2009-09-06 修回日期:2009-09-20 出版日期:2009-12-20 发布日期:2009-12-20
  • 基金资助:

    郑州市科技攻关项目

Identification of Enterococcus from retail Pork and Detection of Virulence factor gene

  • Received:2009-09-06 Revised:2009-09-20 Online:2009-12-20 Published:2009-12-20

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摘要：

2007年～2008年间，分别从郑州市5个农贸市场鲜猪肉销售摊点采集了52份猪肉样品，经细菌分离得到30株疑似肠球菌，通过细菌形态学和培养特性的观察，并采用Vitek-32全自动细菌鉴定系统对其进行分析，最终鉴定为肠球菌，其中粪肠球菌(E. faecalis)16株、屎肠球菌(E. faecium)4株、铅黄肠球菌(E.casseliflavus)6株、鸟肠球菌(E.avium)2株、鹑鸡肠球菌(E. gallinarum )2株。药敏试验结果表明：肠球菌对呋喃妥因、万古霉素、替考拉宁和氨苄青霉素有较高的敏感性，对四环索、卡那霉素和红霉索的敏感性较低。经过对肠球菌6种公认的毒力基因cylA、gelE、efaA、ace、AS和esp的检测，发现分离株携带毒力基因不同，7号粪肠球菌携带6种，而12号屎肠球菌则全部为阴性，其它菌株则携带1～5种不等的毒力基因。

关键词: 绵羊, 绵羊, KIT基因, 克隆, 序列分析

Abstract:

The thirty isolates of enterococcus-like collected from fifty-two raw retail fresh pork in different farm product marker in Zhengzhou city during in 2007~2008, were investigated and identified by observing their morphological and cultivating characteristics and biochemical determination with Vitek-32 system. 16 of 30 isolates were E. faecalis, 4 of 30 isolates were E. faecium, 6 of 30 isolates were E.casseliflavus and eachtwo of 30 isolates was E.avium and E. gallinarum, respectively. Drug sensitivity suggested that all isolates were sensitivity to nitrofurantoin, vancomycin, teicoplanin and ampicillin and majority of isolates were resistance to tetracycline, kanamycin and erythromycin. The known virulence gene of cylA、gelE、efaA、ace、AS and esp for E. faecalis were detected and number of virulence gene among the isolates were different, number 7 possess all virulence gene tested and no virulence gene was detected in number 12, other range from were 1~5,respectively.