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中国农学通报 ›› 2010, Vol. 26 ›› Issue (20): 200-205.

所属专题: 生物技术 玉米

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

施用有机肥对春玉米穗位叶叶肉细胞超微结构的影响

汪仁 邢月华 包红静 隽英华   

  • 收稿日期:2010-03-03 修回日期:2010-05-31 出版日期:2010-10-20 发布日期:2010-10-20

Effects of organic Manure on Mesophyll Cell Ultrastructure of Spring Maize Ear Leaf

  • Received:2010-03-03 Revised:2010-05-31 Online:2010-10-20 Published:2010-10-20

摘要:

研究了施用有机肥对春玉米穗位叶叶肉细胞超微结构的变化。观察发现不施鸡粪的情况下,叶肉细胞内外膜结构模糊;叶绿体膨胀变大,被膜解体,向细胞中央漂移;细胞壁出现褶皱,部分区域出现断裂;线粒体双层膜结构不明显,内脊完全降解;基质片层和基粒片层解体严重。增加鸡粪的施用量,细胞壁逐渐变厚,细胞的“花环型”结构逐渐呈现;施鸡粪 6.7克/公斤土的情况下,细胞壁结构完整,层次清晰,而提高鸡粪用量,细胞壁结构越不完整,结构松散紊乱,细胞壁膨胀变大;施鸡粪 6.7克/公斤土的处理叶绿体膨胀变大,结构紊乱,随着鸡粪施用量的增加,叶绿体呈长椭圆形,被膜逐渐清晰;增加的鸡粪施用量,线粒体双层膜结构越来越明显,内脊数目增多;片层结构逐渐完整紧密,基粒片层和基质片层逐渐清晰可见,基粒片层变密且沿叶绿体长轴方向规律排列。

关键词: 外源腐胺, 外源腐胺, 干旱胁迫, 冬小麦, 膜质过氧化, 有机渗透调节物质

Abstract:

By pot experiment, the changes of mesophyll cell ultrastructure of ear leaf were studied to explore the influence mechanism of organic manure on spring maize early senescence. The observations indicated that without organic manure, the inside and outside membrane structure of mesophyll cell became fuzzy, the chloroplast expanded and moved to cell center, the membrane of chloroplast became disintegrated, the disintegration of stroma and grana lamellae became severe, the double-membrane and inside structure of mitochondrial were unobvious and fuzzy, and the inside ridge degraded absolutely. With increasing of organic manure, the cell wall became thick, the shape of chloroplast became regular and moved to cell edge, the garland-type structure of mesophyll cell appeared,the double-membrane structure of mitochondrial became obvious, the number of inside ridge increased, the lamellae structure became integrated and close, the stroma and grana lamellae became visible and the stroma lamellae arranged regularly along long axis of chloroplast.At rate of 26.7 g/kg, the chloroplast with spindle or oval shape clinged on cell wall, the structure of cell wall and mitochondrial was integrated without appearance of loose phenomenon, the double-membrane structure of mitochondrial was visible, the inside ridge was also visible with much number and the lamellae structure was evidently better than that of other treaments. From the above observations it is concluded that organic manure application could protect cell structure from damage, and the effect was much better with increasing rate, which could prevent spring maize from early senescence.