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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (8): 58-62.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

蓼子朴乙醇提取物对小菜蛾的生物活性研究

林坤 赵堂 杨敏丽 刘文娟   

  • 收稿日期:2010-12-09 修回日期:2010-12-22 出版日期:2011-04-20 发布日期:2011-04-20
  • 基金资助:

    国家自然科学基金资助项目

The Bioactivity of Ethanol Extract from Inula Salsoloides Ostenf. against Plutella xylostella

  • Received:2010-12-09 Revised:2010-12-22 Online:2011-04-20 Published:2011-04-20

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摘要：

为了开发新型、高效植物源农药，探讨蓼子朴作为植物源农药的可能性，笔者在室内测定了蓼子朴乙醇提取物对3龄小菜蛾幼虫的触杀、拒食、产卵忌避、胃毒、抑制生长发育5种生物活性作用。结果表明：蓼子朴乙醇提取物对小菜蛾3龄幼虫有较明显的触杀和拒食活性，校正死亡率和拒食率均随提取物浓度的升高而增大，浸渍法处理小菜蛾3龄幼虫72 h的致死中浓度（LC50）为4.949 g/L，24 h的选择性、非选择性拒食中浓度（AFC50）分别为4.676、4.189 g/L；乙醇提取物对小菜蛾的产卵忌避活性也很好，处理24 h时的忌避效果达70％以上，对小菜蛾的忌避作用随施药后时间的延长而逐渐减弱；乙醇提取物对小菜蛾也有一定的胃毒、抑制生长发育活性。蓼子朴乙醇提取物对小菜蛾3龄幼虫具有较高的触杀和拒食生物活性，故蓼子朴具有潜在的植物源农药开发价值。

关键词: 山东省, 山东省

Abstract:

In order to develop new and high effective plant pesticide, the possibility of Inula Salsoloides Ostenf. as botanical pesticide was investigated. Contacting toxicity, antifeeding, inhibiting oviposition, stomach poisoning and inhibiting growth and development of the ethanol extract from Inula Salsoloides Ostenf. against 3th instar larvae of plutella xylostella were determined in laboratory. The results indicated that the ethanol extract displayed obvious contacting toxicity and antifeeding effect against 3th instar larvae of plutella xylostella. The corrected mortality and antifeedant rate both increased with the increasing of extract concentration. The LC50 of contacting action was 4.949 g/L by dipping method. The choice AFC50 and the non-choice AFC50 were respectively 4.676 g/L and 4.189 g/L at 24 h. The extract displayed good potentials of inhibiting oviposition. The repelling rate was more than 70% at 24 h. The repelling effect gradually decreased as the time increased. The extract also displayed stomach poisoning and inhibiting growth and development on plutella xylostella. The study suggested that the ethanol extract from Inula Salsoloides Ostenf. had higher contacting toxicity and antifeeding against 3th instar larvae of plutella xylostella. Therefore, Inula Salsoloides Ostenf. had potential development value as plant pesticide.