关键词: 澳蜡花, 澳蜡花, 外植体, 组织培养, 污染率

Abstract:

In order to obtain an effective and stable method, the collaborative solid-state fermentation for detoxification of cottonseed meal were processed by adding xylanase, cellulase and mannase, and the parameters of fermentation were optimized in the compound bacillus agent fermenting. The fermentation medium and detoxification conditions were optimized by single-factor test. Then, the optimum composition of the crucial factors of fermentation detoxification was optimized by orthogonal test. The test date was farther analyzed with SPSS 17.0 software. The appropriate conditions of solid fermentation for detoxification of cottonseed meal with the collaborative enzymes were as follows: cottonseed meal: wheat bran=90:10, (NH4)2SO4 1%, complex enzyme 40U/g substrate, fermentation temperature 40℃, the initial pH 6.0, moisture content 50%, the vaccination quantity 6% (W/V), 70 g solid medium in 250 mL triangular flask, fermentation time 6 days. Under the technique conditions, the removal rate of free gossypol reached 96.52%.