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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (2): 65-68.doi: 10.11924/j.issn.1000-6850.2011-2389

所属专题： 水产渔业

• 水产 渔业 • 上一篇    下一篇

生殖季节野生大刺鳅雌雄鱼脂肪酸组成研究

朱定贵   

  • 收稿日期:2011-08-16 修回日期:2011-08-26 出版日期:2012-01-15 发布日期:2012-01-15

Study on the Fatty Acid of the Wild Male and Female Mastacembelus Armatus during Breeding Season

  • Received:2011-08-16 Revised:2011-08-26 Online:2012-01-15 Published:2012-01-15

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摘要：

为更好地开展大刺鳅的人工繁殖。采用气相色谱法分别测定生殖季节野生大刺鳅雌雄鱼性腺、肝胰脏和肌肉的脂肪酸组成与含量。研究结果表明，雌雄鱼各组织的SFA、MUFA含量最高的分别是C16:0、C18:1n-9；PUFA在雌鱼各组织和雄鱼的肝胰脏、肌肉中含量最高的是DHA，在精巢中是C22:4n-6；雌鱼卵巢、肝胰脏和肌肉中，含量最高的脂肪酸分别是C18:1n-9（32.65%）、DHA（18.54%）、C18:1n-9（34.99%）；雄鱼精巢、肝胰脏和肌肉中含量最高的脂肪酸分别是C22:4n-6（33.52%）、DHA（45.68%）、C18:1n-9（23.24%）。大刺鳅亲鱼培育可雌雄鱼分池养殖；在雌鱼饲料中添加C18:1n-9、DHA、ARA，可促进卵巢发育，提高卵子质量；给雄鱼投喂添加DHA、C18:1n-9、ARA和C22:4n-6的饲料，能提高精子质量。

关键词: NaCl胁迫, NaCl胁迫, 网纹甜瓜, 生长量, 叶绿素荧光参数

Abstract:

This investigation means to develop the artificial propagation of Mastacembelus armatus. We used gas chromatography to determine the composition and content of fatty acid in sex gland, hepatopancreas and muscle of the wild male and female during breeding season. The result showed that the highest of SFA and MUFA in various tissues of male and female Marmatus were C16:0 and C18:1n-9; The maximum PUFA in various tissues of female and the hepatopancreas muscle of male was DHA, which appeared C22:4n-6 in the testis. The hightest content of fatty acid in the ovary, hepatopancreas and muscle of female accounted C18:1n-9(32.65%), DHA(18.54%), C18:1n-9(34.99%), respectively. The maximum fatty acid in the testis, hepatopancreas and muscle of male were separately C22:4n-6（33.52%）, DHA（45.68%）, C18:1n-9(23.24%). Therefore, we can cultivate the M. armatus by putting them in 2 different pools: one for female, and the other for male; if the C18:1n-9, DHA, ARA are to be added into the female fodder, we can promote the development of ovary and improve the quality of ovum; while adding the DHA, C18:1n-9, ARA and C22:4n-6 to the male fodder, we can improve the quality of sperm.