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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (10): 116-120.doi: 10.11924/j.issn.1000-6850.2011-2645

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

北京萝卜萎蔫病病原鉴定及病原生长影响因子

耿丽华 迟胜起 张丽 宁利园 史越   

  • 收稿日期:2011-09-13 修回日期:2012-01-09 出版日期:2012-04-05 发布日期:2012-04-05
  • 基金资助:

    国家大宗蔬菜产业技术体系;北京市自然科学基金重点项目;北京市农林科学院科技创新能力建设专项

Identification of the Pathogen of Radish Wilt in Beijing Suburb and Factors Affecting its Growth

  • Received:2011-09-13 Revised:2012-01-09 Online:2012-04-05 Published:2012-04-05

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摘要：

2009年北京郊区发生一种萝卜(Raphanus sativus L.)新病害，其主要症状表现是叶子黄化萎蔫，生长受阻。针对该病的发生，作者采集典型发病植株进行病原菌的分离纯化，用传统的形态学方法和rDNA-ITS序列分析方法进行病原鉴定，并进一步对病原菌生长影响因子进行研究，以便为该病的进一步深入研究、抗病种质资源的筛选和抗病品种的选育打下基础。研究认为该病由大丽轮枝菌(Verticillium dahliae)侵染引起。该病原菌生长适宜温度为20~25℃，适宜pH为6~8，该病原菌能够很好地利用多种氮源和碳源，氮源以硫酸铵最为适宜，碳源则已可溶性淀粉最适宜。这是首次报道由大丽轮枝菌侵染引起的萝卜黄萎病在中国的发生。

关键词: 烘烤, 烘烤, 密集烤房, 炉栅面积

Abstract:

A new radish (Raphanus sativus L.) disease happened in Beijing suburb in 2009. The main symptom of the disease is that the infected radish grows stunt and it’s leaves become yellow. We collected the typical diseased radish plants to isolate the pathogen, and identified the pathogen according to the morphology and the sequence of the internal transcribed spacer region of the ribosomal DNA (ITS), and also studied the major affecting factors of the pathogen in order to provide the basis to study controlling measures and to screen the resistant germplasm resources and select resistant cultivars to the disease. The results were showed as follow: the pathogen of the disease was identified as Verticillium dahliae; The optimal temperature for the pathogen growth was 20-25℃; The optimal pH was 6-8; The pathogen can utilize many kinds of carbon sources and nitrogen nutrient very well, ammonium sulfate was the best nitrogen nutrient and soluble starch was the best of carbon sources for its growth. This was the first report about Verticillium wilt of radish in China.