欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (2): 6-9.doi: 10.11924/j.issn.1000-6850.2011-3158

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

ctsd、ctsl基因的多态性与雪龙黑牛胴体性状相关性研究

陈晓杰 吴蒙 张立敏 陈翠 刘喜冬 周正奎 张猛 黄萌 刘利 高雪 许尚忠 李俊雅 高会江 张路培 李姣   

  • 收稿日期:2011-10-28 修回日期:2011-11-04 出版日期:2012-01-15 发布日期:2012-01-15
  • 基金资助:

    农业部专项; 转基因生物新品种培育重大专项; “十一五”转基因重大专项; 国家发改委雪龙黑牛分子育种关键技术研究及产业化应用

Genetic Polymorphisms of the CTSD and CTSL Gene and Their Association with Carcass Traits in Snow Dragon black Cattle

  • Received:2011-10-28 Revised:2011-11-04 Online:2012-01-15 Published:2012-01-15

PDF (PC)

965

摘要：

为了提高雪龙黑牛的胴体性状，进行新品种选育，本研究以组织蛋白酶D、L(Cathepsin D, Cathepsin L)基因为候选基因，利用PCR-RFLP方法对114头雪龙黑牛F1代商品牛进行多态性检测，应用SAS 9.2软件采用最小二乘拟合线性模型进行分析，结果表明，ctsd，c581t位点与雪龙黑牛的经济性状无明显的相关性，ctsl基因xma I位点与雪龙黑牛的三角牛腩厚显著相关，且AB型个体三角牛腩厚与AA和BB型个体三角牛腩厚差异显著。

关键词: 基因定位, 基因定位

Abstract:

The objective of this study was to explore the genetic polymorphisms of Cathepsin D, L and their association with carcass traits. In this experiment, Genetic polymorphisms of the ctsd and ctsl Genes were studied by PCR-RFLE and analyzed their association with meat quality traits in Snow Dragon black cattle by SAS 9.2. The results showed that ctsd, c581t polymorphism had no associated with economic traits, ctsl, xma I polymorphism had significantly associated with sirloin triangle thickness (P<0.05), sirloin triangle thickness of AB genotype had significant difference with AA and BB genotype.