水稻多蘖矮杆突变体htd7(t)的遗传分析与基因定位

doi:10.11924/j.issn.1000-6850.casb16050072

中国农学通报 ›› 2016, Vol. 32 ›› Issue (33): 138-142.doi: 10.11924/j.issn.1000-6850.casb16050072

所属专题：生物技术；水稻

水稻多蘖矮杆突变体htd7(t)的遗传分析与基因定位

王军,范方军,朱金燕,李文奇,王芳权,仲维功,杨杰

江苏省农业科学院,江苏省农业科学院粮食作物研究所,江苏省农业科学院粮食作物研究所,江苏省农业科学院粮食作物研究所,江苏省农业科学院粮食作物研究所,江苏省农业科学院粮食作物研究所,江苏省农业科学院粮食作物研究所

收稿日期:2016-05-13 修回日期:2016-08-18 接受日期:2016-08-25 出版日期:2016-11-25 发布日期:2016-11-25
通讯作者: 杨杰
基金资助:
：公益性行业科研专项“粮食作物精确定量栽培技术研究与示范”(201303102)；江苏省现代农业重点研发项目“抗穗颈瘟、褐飞虱优良食味粳稻新品种选育”(BE2015355)；国家重大农技推广项目“洪泽县粮食作物新品种、新技术示范基地”(NG[15]003)。

Genetic Analysis and Gene Fine Mapping of A High-tillering and Dwarf Mutant htd7(t) in Rice (Oryza sativa L.)

Received:2016-05-13 Revised:2016-08-18 Accepted:2016-08-25 Online:2016-11-25 Published:2016-11-25

摘要/Abstract

摘要： 分蘖是水稻最重要的农艺性状之一，其决定水稻的最终产量。多蘖矮杆突变体htd7(t)是粳稻品种‘日本晴’经350 Gy 的60Co-γ射线辐射处理后产生的突变体。为了克隆HTD7(t)基因，将htd7(t)与‘9311’配制正反杂交组合进行遗传分析发现，htd7(t)多蘖矮杆性状是受1 对隐性核基因控制。利用SSR分子标记将HTD7(t)初步定位在第11 染色体分子标记RM21 与RM254 之间，遗传距离分别为5.6 cM和3.2 cM。利用已经公布的水稻基因组数据，在该基因附近新发展了13 对InDel 标记，对HTD7(t)进行精细定位。根据定位结果构建覆盖HTD7(t)基因的BAC 重叠群，最终将HTD7(t)定位在InDel11-3 和InDel11-5之间的64.8 kb的物理距离内。

关键词: 艾美耳球虫卵囊, 艾美耳球虫卵囊, 中药, 孢子化率

Abstract: Tillering is one of the most important agronomical characters of rice that determines the final yield. A rice mutant with a high-tillering capacity and dwarf, designated as htd7(t), was isolated from the offspring of the japonica rice (Oryza sativa L. ssp. japonica) variety‘Nipponbare’treated with 350 Gy 60Co-γ radiation. To clone the HTD7(t) locus, positive and negative cross combinations were developed between htd7(t) and‘9311’, genetic analysis found that htd7(t) was controlled by one recessive nuclear gene. HTD7(t) was roughly mapped between two microsatellite markers RM21 and RM254 on the chromosome 11, with genetic distances of 5.6 cM and 3.2 cM, respectively. According to the published rice genome data, 13 new InDel (Insertion-Deletion) markers were developed around HTD7(t), and then fine mapping of HTD7(t) was conducted. The BAC contig of HTD7(t) was constructed based on the mapping results, and HTD7(t) was further mapped between two InDel markers, InDel11-3 and InDel11-5, with physical distance of 64.8 kb.

中图分类号:

S330

王军,范方军,朱金燕,李文奇,王芳权,仲维功,杨杰. 水稻多蘖矮杆突变体htd7(t)的遗传分析与基因定位[J]. 中国农学通报, 2016, 32(33): 138-142.

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水稻多蘖矮杆突变体htd7(t)的遗传分析与基因定位

Genetic Analysis and Gene Fine Mapping of A High-tillering and Dwarf Mutant htd7(t) in Rice (Oryza sativa L.)

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