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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (15): 260-263.doi: 10.11924/j.issn.1000-6850.2011-3232

所属专题： 小麦

• 食品 营养 检测 安全 • 上一篇    下一篇

苦荞麦黄酮含量萃取工艺的研究

施卫省 罗小林 曹新佳 许邦丽   

  • 收稿日期:2011-11-03 修回日期:2011-12-27 出版日期:2012-05-25 发布日期:2012-05-25

Study on the Technology of Extraction of Flavonoids Content in the Bitter Buckwheat

  • Received:2011-11-03 Revised:2011-12-27 Online:2012-05-25 Published:2012-05-25

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摘要：

为了研究其总黄酮的提取工艺，以苦荞麦不同器官为原料，采用高压提取、超声波萃取、高压提取+超声波萃取混合萃取苦荞麦黄酮含量。结果表明：高压15 min，测定黄酮含量：叶片5.46%、茎秆1.27%、根系0.54%；超声波萃取为60 min，测定黄酮含量：叶片5.72%、茎秆1.71%、根系0.84%；实施高压 + 超声波混合提取，以高压10 min + 超声波30 min，提取的黄酮含量最高：其叶片黄酮含量的平均值为6.34%，比相同条件的高压提取(5.07%)增加1.27%，比相同条件的超声波萃取(5.53%)增加0.81%。高压10 min+超声波30 min的回收率最高，为96.7%。

关键词: 国兰, 国兰, 大花蕙兰, 杂交育种, 无菌播种

Abstract:

This text studied on the methods of extracting flavonoids of different organs on the bitter buckwheat as raw materials. The method was the high pressure extraction and the Ultrasonic extraction and the mix high pressure and the Ultrasonic extraction. The results showed that, in high pressure extraction 15 min, the content of flavonoids in the leaf was 5.46% and that of stem was 1.27%, and that of root was 0.54%. In the Ultrasonic extraction 60 min, the content of flavonoids in the leaf was 5.72% and that of stem was 1.71%, and that of root was 0.84%. In mix high pressure and the Ultrasonic extraction, the content of flavonoids was the highest in high pressure 10 min + the Ultrasonic extraction 30 min. The content of flavonoids of leaf was 6.43% and that was better than the average of the same conditions in high pressure extraction (5.07%) and that increased by 1.27%, and that was better than the average of the same conditions in the Ultrasonic extraction (5.53%), and that increased by 0.81%. The conclusion was that, the rate of recovery was the highest and that was up to 96.7% in high pressure 10 min + the Ultrasonic extraction 30 min.