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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (13): 65-69.doi: 10.11924/j.issn.1000-6850.2011-3490

• 林学 园艺 园林 • 上一篇    下一篇

杂种小叶杨叶片外植体离体再生体系的建立

付文锋 姜鹏 张耀 奚晓军 张茜 赵健 张金凤   

  • 收稿日期:2011-11-22 修回日期:2012-02-12 出版日期:2012-05-05 发布日期:2012-05-05
  • 基金资助:

    石羊河下游荒漠绿洲过渡带固沙植被退化与土壤演变的数量关系研究;教育部科学技术研究重点项目

In Vitro Rapid Multiplication and Propagation from Leaves of P. simonii Carr.×P. deltoides cv. ‘Shanhaiguanensis’

  • Received:2011-11-22 Revised:2012-02-12 Online:2012-05-05 Published:2012-05-05

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摘要：

为了开发新的小叶杨基因保存技术并提高其抗病虫害能力，本研究以杂种小叶杨(Populus simonii Carr.× P. deltoides cv. ‘Shanhaiguanensis’)无性系的叶片作为外植体，研究了杂种无性系的的离体培养及叶片再生体系，探讨了不同激素组合对杂种无性系不定芽的诱导、分化以及生根的影响。结果表明：最适于杂种小叶杨叶片分化的培养基是MS+0.1 mg/L 6-BA+0.04 mg/L NAA+0.005 mg/L TDZ，不定芽分化率和平均分化芽芽数分别达到最高的90%和6.8，芽长为2.14 cm；最佳生根培养基为1/2 MS+0.1mg/L IBA，生根率达90%。最后将这些再生植株成功驯化并移栽到温室。10 mg/L的卡那霉素可以抑制杂种小叶杨的诱导分化，20 mg/L的卡那霉素可以抑制杂种小叶杨不定芽的生根。本研究结果将有助于小叶杨抗病虫害等方面的遗传转化及相关研究，同时也将为小叶杨基因库的保存提供重要技术支撑。

关键词: 幼苗生长, 幼苗生长

Abstract:

In order to develop new technology for Populus simonii gene preservation and enhance its resistance to disease and pests, leaves of hybrid clone of P. simonii Carr.×P. deltoides cv. ‘Shanhaiguanensis’ were used as explants to study it’s in vitro culture and leaf regeneration system, and to explore the effect of different prescriptions of hormone on induction, differentiation of adventitious bud, and rooting. The results showed that: the optimum medium for adventitious buds regeneration of P. simonii Carr.×P. deltoides cv.‘Shanhaiguanensis’ was MS+0.1 mg/L 6-BA+0.04 mg/L NAA+0.005 mg/L TDZ with the maximum adventitious bud frequency (90%), number of shoots (6.8), shoot length (2.14 cm). The optimum medium for rooting of shoots was 1/2 MS + 0.1 mg/L IBA with a rooting efficiency of 90%. The plantlets thus obtained were successfully hardened and transplanted to greenhouse. 10 mg/L kanamycin could inhibit the bud regeneration of “P. simonii Carr.×P. deltoides cv. ‘Shanhaiguanensis’”, 20 mg/L kanamycin could inhibit the root regeneration of P. simonii Carr.×P. deltoides cv. ‘Shanhaiguanensis’ from the shoots. The results of this study will not only contribute to the pest-resistant area of P. simonii and the related studies of genetic transformation, but also contribute to provide important technical support for the preservation of gene pool of P. simonii.

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