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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (13): 215-220.doi: 10.11924/j.issn.1000-6850.2011-3503

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

草莓SBP基因家族生物信息学初步分析

葛安静 张春华 董清华 宋长年 张希 赵密珍   

  • 收稿日期:2011-11-24 修回日期:2012-02-24 出版日期:2012-05-05 发布日期:2012-05-05
  • 基金资助:

    北京市教委科技发展计划项目

Primary Bioinformatics Analysis of the SBP Gene Family in Strawberry

  • Received:2011-11-24 Revised:2012-02-24 Online:2012-05-05 Published:2012-05-05

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摘要：

为了研究草莓SBP基因家族的功能，利用多种生物信息学软件，对SBP蛋白序列的系统发生和基因组定位进行分析，对其氨基酸组成成分、理化性质以及二、三级结构进行分析和预测等。结果表明草莓16条SBP蛋白序列与拟南芥16条SBP蛋白序列一起分成了3个亚族，说明拟南芥与草莓SBP基因间具有较高的保守性。基因组定位发现16个SBP基因分布在6条染色体上。研究还发现不同亚族间氨基酸数目、氨基酸序列间的疏水性存在一定的差异，而它们的二级结构主要以随机卷曲为主，三级结构相似。SBP基因家族在植物的生长、发育等多个方面起重要作用，本实验结果为草莓SBP基因家族的进一步功能分析提供了重要研究基础。

关键词: 栽培技术, 栽培技术

Abstract:

The aim was to study the function of SBP gene family in strawberry. Various bioinformatics methods were used to analyze the phylogenesis of SBP protein sequences and the localization of SBP genome, their amino acid composition, physical and chemical characteristics, as well as secondary and tertiary structures. The result showed that these 16 amino acid sequences could be clustered into 3 groups together with 16 SBP sequences of Arabidopsis thaliana, which illuminated that SBP genes had highly conservative between strawberry and Arabidopsis thaliana. Further genetic mapping of SBP genome localization found that they could be distributed on 6 chromosomes. The research still found that the number of amino acid and hydrophobic of amino acid sequences in different subfamilies presented some differences, however, the results of secondary structure predicted that the main composition of 16 amino acid sequences was random curl and the tertiary structure of all the sequences were quite similar. SBP gene family plays great role in plant growth and development. The results of this investigation could definitely provide a significant foundation for further research on function analysis of SBP gene family in Strawberry.