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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (14): 31-36.doi: 10.11924/j.issn.1000-6850.2012-2708

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

PPARα和PPARγ的克隆表达研究进展

刘寒 李苏 赵玉华 王卫民   

  • 收稿日期:2012-08-03 修回日期:2012-09-11 出版日期:2013-05-15 发布日期:2013-05-15
  • 基金资助:
    现代农业产业技术体系建设专项;中央高校基本科研业务费专项资金

Advance in the clone and expression of PPARα and PPARγ

Weimin Wang   

  • Received:2012-08-03 Revised:2012-09-11 Online:2013-05-15 Published:2013-05-15

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摘要： 过氧化物酶体增殖物激活受体(peroxisome proliferator-activated receptors，PPARs)是一种配体激活的核受体转录因子，属于核激素受体超家族。该因子与其相应的配体结合后能与目标基因启动子内的反应元件相结合，从而调节核内目标基因的表达。PPARs自首次在小鼠中被克隆出来以来，在人类、啮齿类、两栖类及鱼类中被相继克隆。PPARs因其在糖脂类代谢，细胞分化、凋亡，炎症反应等中起重要作用，因此被广泛应用于疾病的研究中。简述了PPARs结构与分类及作用机理，归纳了PPARs参与脂肪代谢、糖代谢及炎症反应等主要生物学功能，并着重总结了哺乳动物、鱼类及胚胎发育中PPARα和PPARγ基因克隆表达情况，最后对PPARs研究中存在的问题及研究前景进行了展望。

关键词: 幼苗生长, 幼苗生长

Abstract: Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear receptor superfamily of transcription factors respond to specific ligands. PPARs molecule binds to the specific ligands, then binds to peroxisome proliferator response elements (PPRE) on DNA, regulating the expression of the target gene. Since PPARs was first cloned in rats, they had been cloned in human, rodents, amphibian, and fish. PPARs played a crucial role in glucose and lipid metabolism, cell differentiation, apoptosis, and inflammation. Thus, PPARs had been applied to the study of diseases. This paper briefly reviewed the structure, classification, and mechanism of PPARs. The main biological functions including lipid metabolism, glucose metabolism, and inflammation were summed up. Furthermore, the clone and expression of PPARα and PPARγ in mammal, fish and embryonic development were extraordinarily discussed. A brief review on the problem and the research trend of PPARs was also discussed in this paper.