欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (21): 179-183.doi: 10.11924/j.issn.1000-6850.2012-2993

• 植物保护 农药 • 上一篇    下一篇

安徽省4种寄主南方根结线虫鉴定

姚洁 陈良宏 吴慧平 王龙平 崔强   

  • 收稿日期:2012-09-03 修回日期:2012-10-10 出版日期:2013-07-25 发布日期:2013-07-25

Identification and Comparision of Root-knot Nematodes from Four Hosts in Anhui

  • Received:2012-09-03 Revised:2012-10-10 Online:2013-07-25 Published:2013-07-25

PDF (PC)

633

摘要： 为了鉴定安徽省栝楼(Trichosanthes kirilowii)、桔梗(Platycodon grandiflorum)、木耳菜(Basella rubra)和小叶黄杨(Buxus sinica)上的根结线虫(Meloidogyne spp.)种类，并比较不同RKN种群间形态差异，运用形态学和酯酶同工酶凝胶电泳特征条带分析方法对安徽省4种寄主南方根结线虫进行种类鉴定。形态学观察和酯酶同工酶凝胶电泳特征条带分析结果均表明：栝楼、桔梗和木耳菜上的RKN均为南方根结线虫(Meloidogyne incognita)；形态学观察结果表明：小叶黄杨上的根结线虫肥西种群为南方根结线虫(M. incognita)疑似种，酯酶同工酶凝胶电泳特征条带分析结果将其确定为南方根结线虫(M. incognita)，木耳菜和小叶黄杨上发生的南方根结线虫为安徽省新种。该研究结果为本地根结线虫防治、南方根结线虫生理分化研究、种群和寄主间关系研究提供依据。

关键词: 效应, 效应

Abstract: To identify root-knot nematodes (RKN) isolated from Trichosanthes kirilowii , Platycodon grandiflorum , Basella rubra ,Buxus sinica in Anhui Province, compared morphological differences among RKN population, and applied morphological method and esterase isozymes characteristic striped analysis method to identify RKN populations. The results of morphological observation and esterase isozymes characteristic striped analysis showed RKN onT. kirilowii, P. grandiflorum , B. rubra wereMeloidogyne incognita. The morphological observation showed RKN onB. sinica wasM. incognita suspected kinds, this result was confirmed by esterase isozymes characteristic striped analysis.M. incognita onB. rubra andM. incognita onB. sinica were new species in Anhui Province. The results of the study were basis for RKN disease control in the local area, the research of physiological differentiation ofM. incognita and the relationship between the population and host.