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中国农学通报 ›› 2013, Vol. 29 ›› Issue (7): 20-25.doi: 10.11924/j.issn.1000-6850.2012-3620

所属专题: 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

菲油果自然越冬条件下的生理指标变化的研究

唐丽 张瑜 袁德义   

  • 收稿日期:2012-11-06 修回日期:2012-11-25 出版日期:2013-03-05 发布日期:2013-03-05
  • 基金资助:
    “948” 引进国际先进林业科学技术项目 “菲油果品种及栽培新技术引进” (2008-4-06)。

The Study of Physiological Indexes of Feijoa sellowiana Berg in Over-winter Low-temperature Environment

  • Received:2012-11-06 Revised:2012-11-25 Online:2013-03-05 Published:2013-03-05

摘要: 为了探讨菲油果的抗寒特性,以引种的Coolidge和Mammoth 2个菲油果新品种成熟叶为试验材料,在自然越冬低温环境条件下,对不同低温条件下叶片电导率、可溶性糖、游离脯氨酸(Pro)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、丙二醛(MDA)等几个生理指标的变化情况进行研究。结果表明:在一定的温度范围内,不同品种的菲油果叶片的电导率均随着温度的降低而增大;可溶性糖含量和游离脯氨酸(Pro)随着温度的降低而增加。自然越冬过程中不同品种的菲油果的游离脯氨酸(Pro)呈有规律的变化;细胞内的保护酶(SOD、POD)都表现为随着温度的降低,其活性增强。方差分析结果显示,不同品种的菲油果相对电导率、丙二醛(MDA)、游离脯氨酸(Pro)、过氧化物酶(POD)差异达到显著水平,不同温度间电导率、可溶性糖、丙二醛(MDA)、游离脯氨酸(Pro)、过氧化物酶(POD)、超氧化物歧化酶(SOD)差异达到极显著水平;不同品种间的超氧化物歧化酶(SOD)差异不显著。

关键词: 组织培养, 组织培养

Abstract: In order to study the cold resistance characteristics of Feijoa sellowiana Berg, the author used the mature leaves of Feijoa sellowiana Berg, Coolidge and Mammoth in over-winter low temperature environment as test materials, by researching on several physiological indexes: the electrical conductivity, soluble sugar and free proline (Pro), superoxide dismutase (SOD), peroxidase (POD) and malondialdehyde (MDA). The results showed that: in a certain temperature range, the electrical conductivities, the content of soluble sugar and free proline (Pro) of different species of Feijoa sellowiana Berg were increasing with temperatures decreasing. In the over-winter process, the free proline (Pro) of different kinds of Feijoa sellowiana Berg had a regular change. The activity of protective enzymes (SOD, POD) inside the cells were enhancing with temperatures reduced. Variance analysis results showed that: variance differences of relative conductivity, malondialdehyde (MDA), free proline (Pro), peroxidase (POD) between different species reached significant level. And the electrical conductivity, soluble sugar, malondialdehyde (MDA), free proline (Pro), peroxidase (POD) and superoxide dismutase (SOD) indexes reached extremely significant level between different temperatures. There was no significant difference between the superoxide dismutase (SOD) in different species.