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中国农学通报 ›› 2013, Vol. 29 ›› Issue (28): 110-114.doi: 10.11924/j.issn.1000-6850.2013-0383

所属专题: 园艺

• 林学 园艺 园林 • 上一篇    下一篇

不同抗寒性葡萄根系导管分子形态观察

王浩 张京政 谢兆森 齐永顺   

  • 收稿日期:2013-02-04 修回日期:2013-03-15 出版日期:2013-10-05 发布日期:2013-10-05
  • 基金资助:
    河北省科技厅项目

Morphological Observation on Root Vessel Elements of Different Cold Resistance Grape Cultivars

  • Received:2013-02-04 Revised:2013-03-15 Online:2013-10-05 Published:2013-10-05

摘要: 为探究不同抗寒性葡萄品种根系导管分子形态差异,分别对6种葡萄根系低温处理后,进行根段培养,鉴定6种葡萄根系抗寒力。采用组织离析[1]和显微照相技术,分别对6种葡萄根系导管分子进行观察。试验结果表明:葡萄根系中含有多种类型导管,每种导管长度、直径、两端斜度、具尾情况各不相同。不同类型的导管分子在不同品种葡萄中所占比例不同,在抗寒品种中,长度较长、直径大、两端斜度小、单尾或无尾的导管所长比例显著高于高非抗寒品种。在长度比较中,导管长度450 μm以上时,抗寒品种导管比例比非抗寒品种高4.9%~13.6%;在直径比较中,导管直径在0~50.0 μm范围内,抗寒品种导管比例比非抗寒品种低12.4%~30.1%。导管直径在100 μm以上时,抗寒品种导管比例比非抗寒品种高25.0%~29.5%。从导管斜度比较看,两端倾斜的导管中,在30°—45°范围内抗寒品种导管比例比非抗寒品种低20%~27%;在45°以上时,抗寒品种导管比例为0%,非抗寒品种导管比例为4%~5%;从具尾情况看,在两端无尾导管类型中,抗寒品种导管所占比例比非抗寒品种高18%~25%,在两端具尾导管类型中,抗寒品种导管所占比例比非抗寒品种低10%~13%。

关键词: 品质, 品质

Abstract: In order to make clear the morphological difference of root vessel elements of six grape cultivars with different cold resistance, the cold resistance power of the root from 6 grape cultivars were studied after low temperature treatment and root segment culture. We used tissue segregation procedure and microphotography method to study the morphological difference of the root vessel elements. The results showed that the root vessels were different in type the size and slope for the end walls and tails; different cultivars of grape had different types of vessel elements, and the proportion of different types of vessel elements was also different; the proportion of long-length, big-inch, one tail or no tail, small-slope of both end walls vessel elements in cold-resistance cultivars significantly higher than non-cold resistance cultivars; the proportion of vessel element which was more than 450 μm in length owned a 4.9% to 13.6% higher for the cold resistance cultivars vessel elements than non-cold resistance cultivars; the proportion of the diameter of the vessel element which was less than 50.0 μm, was 12.4%-30.1% lower for the cold resistance cultivars vessel elements than non-cold resistance cultivars, but the proportion of vessel element diameter which was more than 100 μm owned a 25.0%-29.5% higher for cold resistance cultivars than non-cold resistance cultivars; the proportion of cold resistance cultivars vessel elements was 20%-27% lower than non-cold resistance’ in the range of end wall slope angle from 15° to 30°, but the proportion of cold resistance cultivars vessel elements was 0% for the slope angle more than 45°, while the non-cold resistance was 4%-5%; the proportion of cold resistance cultivars vessel elements owned a 18%-25% higher than non-cold resistance cultivar for the type of no tail in one wall, while the proportion of cold resistance cultivars vessel elements was 10%-13% lower than non-cold resistance cultivar for the type which both end walls had tails.

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