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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (1): 178-184.doi: 10.11924/j.issn.1000-6850.2013-1075

• 林学 园艺 园林 • 上一篇    下一篇

渝产白芷高效液相色谱指纹图谱研究

谭秋生 章文伟 李 玲 邓才富 罗 舜 罗 川   

  • 收稿日期:2013-04-15 修回日期:2013-06-04 出版日期:2014-01-05 发布日期:2014-01-05
  • 基金资助:
    重庆市卫生局中医药科技项目 “渝产白芷高效液相色谱指纹图谱的建立及质量研究” (2010-2-162)。

Research on the HPLC Fingerprint of Radix Angelica dahurica in Chongqing

  • Received:2013-04-15 Revised:2013-06-04 Online:2014-01-05 Published:2014-01-05

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摘要： 建立渝产白芷高效液相色谱指纹图谱，并分析其质量的主要影响因子，以便规范渝产白芷科学发展和有效控制其生产质量。以RP-HPLC法采集色谱图，用国家药典委员会“中药色谱指纹图谱相似度评价系统（2004年）”建立各类对照指纹图谱并计算相似度。结果表明：65批次白芷样品指纹相似度介于0.633~0.997，其中9批次介于0.633~0.773，均为熏硫品，其余都超过了0.9；渝产白芷与遂宁白芷相似性很强；渝产白芷的指纹相似度，以产地、种植土壤和采收加工方法影响最大，海拔高度差异影响其次，采收日期和表皮差异影响最小。

关键词: 农业, 农业

Abstract: The purpose of this research was to establish a HPLC fingerprint and analyse its main factors of Angelicae dahuricae in Chongqing in order to regulate the development and control quality. In the research, chromatogram collected by RP-HPLC method, the “Similarity Evaluation System for Chromatographic Fingerprint of TCM”published by GPC (Version 2004) was used to establish the various reference fingerprint and evaluate its similarity. The result showed that fingerprint similarity of 65 lots between 0.633 to 0.997, 9 lots ranged from 0.633 to 0.773 which treated with smouldering sulphur, others were all over 0.9; the quality of Angelica dahurica in Chongqing was similar with Suining area; the most influencing factors of fingerprint similarity in Chongqing were area、soil type and processing method, then altitude, harvesting time and types of skin had the least influence.