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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (7): 82-85.doi: 10.11924/j.issn.1000-6850.2013-1691

所属专题： 食用菌； 食用菌

• 林学 园艺 园林 • 上一篇    下一篇

白灵菇高活力液体菌种培养条件研究

董玉兰 陈艳光 李书生 张丽萍 程辉彩 张根伟   

  • 收稿日期:2013-06-19 修回日期:2013-07-25 出版日期:2014-03-05 发布日期:2014-03-05
  • 基金资助:
    河北省科技公关项目“松蘑规模化出菇技术研究”(09235502D)；河北省科学院科技计划项目“食用菌栽培污染综合防治技术研究与示范”(11326)。

The Study of High Energy Liquid Culture Conditions of Pleurotus nebrodensis

  • Received:2013-06-19 Revised:2013-07-25 Online:2014-03-05 Published:2014-03-05

PDF (PC)

498

摘要： 为了提高白灵菇液体菌种的活力，通过采用单因子和正交发酵试验方法，以白灵菇菌丝生物量、菌球密度及萌发活力指数为检测指标，研究白灵菇高活力液体菌种培养基配方。结果表明，该菌株的最适碳源为玉米粉，最适氮源为黄豆粉和蛋白胨，最佳培养基碳氮配方为玉米粉3%，葡萄糖1.5%，蛋白胨0.6%，黄豆粉2%。

关键词: HPLC, HPLC

Abstract: The appropriate liquid fermentation medium were studied by measuring the mycelial biomass, the peloton density and the amount of germination vigor index and the single factor tests and orthogonal design method were employed to enhance the vitality of Pleurotus nebrodensis strains of liquid. The results showed that, the optimal suitable carbon source were corn flour, the optimum nitrogen source were soybean meal and peptone, the optimum medium were 3% of the corn flour, glucose1.5%, peptone 0.6%, soybean meal 2%.