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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (15): 11-16.doi: 10.11924/j.issn.1000-6850.2013-2972

• 农学 农业基础科学 • 上一篇    下一篇

“神舟八号”搭载‘航恢173’种子的当代生物效应及SSR分析

罗文龙 陈立凯 王慧 刘永柱 郭涛 陈志强   

  • 收稿日期:2013-11-13 修回日期:2013-11-29 出版日期:2014-05-25 发布日期:2014-05-25
  • 基金资助:
    国家 “863” 计划资助项目(2012AA101201)； 广东省科技计划项目(2011A020102004)。

Biological Effects and SSR Analysis on M1 Generation of ‘Hanghui 173’ (Oryza sativa L. ssp. indica) Whose Seeds Carried in the "Shenzhou Ⅷ" Spacecraft

  • Received:2013-11-13 Revised:2013-11-29 Online:2014-05-25 Published:2014-05-25

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摘要： 水稻空间诱变的当代效应与地面其他常用的理化诱变方法存在较大差别，并且不同航天器的诱变效应往往也不尽一致。为了探明搭载“神舟八号”飞船的当代效应及其特征，以籼稻‘航恢173’种子经“神舟八号”搭载诱变当代(SP1)为材料，考察其发芽率、苗高、成熟期株高、剑叶长和宽、主穗长和结实率等7个性状，探讨“神舟八号”搭载的当代效应，并与250 Gy 60Co-γ射线辐照当代(G1)和1% EMS诱变处理当代(E1)进行对比；进一步利用位于水稻12条染色体的24个SSR标记对100个混合SP1样品（300个单株）进行变异检测，以期从分子水平了解“神舟八号”搭载的诱变效应。结果表明，与对照相比，SP1的发芽率、主穗结实率分别显著和极显著下降，而其余5个性状差异不显著；对比分析表明，与“神舟八号”搭载对植株后期生长无明显影响不同的是，G1和E1的植株其后期生长受到促进，导致G1、E1和SP1在株高、剑叶长和宽上均存在显著或极显著差异。SSR座位的扩增结果表明，仅SSR标记RM267在1个样品中检测到明显的变异条带，变异频率约为0.014%。

关键词: 水稻, 水稻, NaCl, 发芽率, 发芽势

Abstract: Effects on M1 generation of rice induced by space-flight was quite different from that induced by other commonly used physical and chemical mutagens, and even the effects of M1 populations carried in different space-crafts were not exactly the same. To investigate the effects on M1 generation of rice cultivar‘Hanghui 173’(Oryza sativa L. ssp. indica) whose seeds were carried in "ShenzhouⅧ" spacecraft (SP1), seven characters including the germination rate, seedling height, plant height, length and width of flag-leaf, length and setting percentage of main-panicle were determined, and comparison of the SP1 to 250 Gy 60Co-γrays irradiated- M1 generation (G1) and 1% EMS (Ethyl Methane Sulphonate) treatment - M1 generation (E1) was conducted. Further, to study the molecular mutagenic effect of "ShenzhouⅧ" carrying, 24 SSR markers were used to screening genetic variations within 100 mixed samples from 300 SP1 individuals. The results showed that, contrast to the control, germination rate and main-panicle's setting percentage of the SP1 decreased at significant and highly significant respectively, while remained five characters were found no significant difference. Comparative analysis showed that the "ShenzhouⅧ" carrying did not have effects on the late growth of SP1 plants, whereas the late growth of G1 and E1 plants were promoted leading to significant or highly significant differences on their plant height and length and width of flag-leaf. Amplification of 24 SSR markers showed that only one variation band was detected at locus RM267, and the corresponding variation rate was 0.014%.