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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (26): 27-32.doi: 10.11924/j.issn.1000-6850.2013-3055

所属专题： 生物技术； 资源与环境

• 水产 渔业 • 上一篇    下一篇

基于ITS序列海带目种质资源的物种鉴定研究

崔翠菊   

  • 收稿日期:2013-11-20 修回日期:2013-12-26 出版日期:2014-09-15 发布日期:2014-09-15
  • 基金资助:
    国家863重大项目“基于全基因组信息的藻类遗传选育”(2012AA10A406)；国家科技支撑计划“海藻栽培新种质苗种繁育与栽培技术研究及产业化示范”(2012BAD55G01)；烟台市科技发展计划项目“药用和食用海藻新种质苗种繁育与栽培技术研究及产业化示范”(2013LGS002)。

Identification of Laminariales Germplasm Resources Based on Ribosomal DNA Internal Transcribed Spacer (ITS) sequence

  • Received:2013-11-20 Revised:2013-12-26 Online:2014-09-15 Published:2014-09-15

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摘要： 为了更准确地鉴定海带目种质资源，为种质保存和保护提供依据，研究采用海带目的核糖体DNA内转录间隔区（Internal Transcribed Spacer，rDNA ITS区）的通用引物，以待鉴定海带目样品的基因组DNA为模板通过PCR方法扩增出ITS序列，在测序的基础上对25棵分别采自日本和烟台海区的海带目样品进行分子鉴定。结果表明，25个待鉴定褐藻目样品和16个已知物种，通过ITS序列聚类分析，分别聚在不同的单系类群中且在物种鉴定树中种内的支持率均较高，分属于2科3属，聚类为7个不同的种，形态学观察的结果与分子鉴定结论相吻合。海带目ITS序列引物通用性强，有效扩增率高，可用于海带目物种的快速鉴定。

关键词: 品质, 品质

Abstract: The aims were to identify Laminariales germplasm resources accurately and provide the basis for germplasm conservation and protection. The universal primers of ribosomal DNA Internal Transcribed Spacer (Internal Transcribed Spacer of rDNA, ITS) were adopted to amplify the ITS sequences of the undetermined samples by PCR, on the basis of the sequencing, the molecular identification was carried out among 25 samples collected from Japan and Yantai sea area. The results showed that 25 undetermined samples and 16 known species were clustered in different groups by clustering analysis of ITS sequence. Within the species identification trees, the ratings are high. The 25 undetermined samples were clustered in three genera, two families and seven different species respectively. The results of morphological observation conformed to the molecular identification. The primers of Laminariales ITS sequence could be used in rapid species identification as its versatility and high efficient amplification rate.