桑叶提取物1-DNJ对小菜蛾幼虫生物活性的影响

doi:10.11924/j.issn.1000-6850.2013-3370

中国农学通报 ›› 2014, Vol. 30 ›› Issue (19): 293-297.doi: 10.11924/j.issn.1000-6850.2013-3370

所属专题：园艺

桑叶提取物1-DNJ对小菜蛾幼虫生物活性的影响

张秀丽王炎徐东刘斌敖红张会慧

收稿日期:2013-12-26 修回日期:2014-02-19 出版日期:2014-07-05 发布日期:2014-07-05
基金资助:
中央高校基本科研业务费专项资金项目“桑叶浸提液对菜青虫生长的干扰机理及活性成分的分离”(DL11BA14)；国家自然科学基金“桑树提取物生物活性成分及其杀虫机理研究”(31070307)。

Effect of 1-DNJ from Mulberry Leaves on the Biological Activity of Plutella xylostella L.

Received:2013-12-26 Revised:2014-02-19 Online:2014-07-05 Published:2014-07-05

摘要/Abstract

摘要： 为研究桑树叶片生物活性物质1-DNJ的杀虫作用机理，以小菜蛾(Plutella xylostella L.)为研究对象，利用桑树叶片提取物1-DNJ饲喂小菜蛾3龄幼虫，研究1-DNJ对小菜蛾拒食、触杀、胃毒和生长等生物活性的影响。结果表明，1-DNJ处理后，小菜蛾48 h和72 h的拒食率分别是93.33%和89.25%，拒食中浓度为0.34 g/L和0.58 g/L。同时，1-DNJ对小菜蛾幼虫的生长发育具有明显抑制，小菜蛾化蛹率随着1-DNJ浓度增加而显著下降，但对小菜蛾的蛹重作用不大。1-DNJ对小菜蛾的触杀和胃毒作用效果没有拒食和生长抑制的作用明显。结果说明，桑树叶片中的1-DNJ主要通过拒食作用影响小菜蛾生长，进而达到防控的目的。

关键词: 华北暴雪, 华北暴雪, 微物理, 微物理降水形成机制

Abstract: Bioassays in laboratory were employed to investigate anti-feeding, growth inhibitory functions of 1-DNJ extracted from mulberry leaves on the 3th Plutella xylostella. Results showed that the highest anti-feeding rates were 93.33 and 89.25% respectively at 24 and 48 h, and the AFC50 were 0.34 and 0.58 g?L-1, respectively. The developmental inhibition assay indicated that weight and pupatation rates decreased with the increasing 1-DNJ concentration. While, the pupal weight of P. xylostella was non-distinctive inhibited. However, the mortality rates of contact toxicity and stomach toxicity were less than anti-feeding and growth inhibitory effect. These results implied that mulberry was treasuries of bioactive substances useful as pesticides

张秀丽王炎徐东刘斌敖红张会慧. 桑叶提取物1-DNJ对小菜蛾幼虫生物活性的影响[J]. 中国农学通报, 2014, 30(19): 293-297.

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桑叶提取物1-DNJ对小菜蛾幼虫生物活性的影响

Effect of 1-DNJ from Mulberry Leaves on the Biological Activity of Plutella xylostella L.

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