一种简单快速测定对虾白斑综合症病毒浓度方法的建立

doi:10.11924/j.issn.1000-6850.2014-0583

中国农学通报 ›› 2014, Vol. 30 ›› Issue (20): 30-34.doi: 10.11924/j.issn.1000-6850.2014-0583

所属专题：水产渔业

一种简单快速测定对虾白斑综合症病毒浓度方法的建立

秦崇涛

收稿日期:2014-03-07 修回日期:2014-05-06 出版日期:2014-07-15 发布日期:2014-07-15
基金资助:
国家自然科学基金 “对虾白斑综合症病毒（WSSV）主要囊膜蛋白相互作用位点的鉴定” (31272698)。

Evaluate the Concentration of Purified White Spot Syndrome Virus with Spectrophotometry

qin chongtao

Received:2014-03-07 Revised:2014-05-06 Online:2014-07-15 Published:2014-07-15

摘要/Abstract

摘要： 测定出对虾白斑综合症病毒（WSSV）中基因组DNA在WSSV总质量的比例，再根据WSSV的基因组大小计算出其质量，计算出完整的WSSV病毒颗粒的质量为3.079×10^-15 g。同时测定了各个不同组分在WSSV干重中所占的比例：核衣壳包括基因组DNA和蛋白质，其比例分别为10.17%和43.07%，囊膜包括膜蛋白和各种脂类物质，比例分别为24.76%和22%；测定出WSSV悬液中WSSV浓度与其OD₆₀₀的换算关系为C=OD₆₀₀×6.79×10⁸个/μL。

关键词: 橡胶树, 橡胶树

Abstract: First the quality of WSSV genome was calculated and the genomic DNA ratio of total WSSV particle mass was measured. According to the calculation, the mass of complete WSSV particles was 3.079×10^-15g. In addition, the different components’proportion in WSSV dry weight was determined. The genomic DNA accounted for 10.17%, nucleocapsid accounted for 43.07%, envelop lipid accounted for 22%, envelop protein accounted for 24.76%. The relation between the concentration of WSSV and the OD₆₀₀ of the suspension of WSSV is C=OD₆₀₀×6.79×108virions/μL。

秦崇涛. 一种简单快速测定对虾白斑综合症病毒浓度方法的建立[J]. 中国农学通报, 2014, 30(20): 30-34.

qin chongtao. Evaluate the Concentration of Purified White Spot Syndrome Virus with Spectrophotometry[J]. Chinese Agricultural Science Bulletin, 2014, 30(20): 30-34.

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一种简单快速测定对虾白斑综合症病毒浓度方法的建立

Evaluate the Concentration of Purified White Spot Syndrome Virus with Spectrophotometry

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