蝉蜕诱导对球孢白僵菌生物学特性及其毒力影响的研究

doi:10.11924/j.issn.1000-6850.2014-1289

中国农学通报 ›› 2014, Vol. 30 ›› Issue (34): 51-55.doi: 10.11924/j.issn.1000-6850.2014-1289

蝉蜕诱导对球孢白僵菌生物学特性及其毒力影响的研究

关兵兵¹，王冰¹，刘艳微¹，孟鑫睿¹，李晓慧¹，张佳诗²，
徐文静²，李启云²，冯树丹¹，张正坤²

（¹哈尔滨师范大学，哈尔滨 150025；²吉林省农业科学院植物保护研究所，吉林公主岭 136100）

收稿日期:2014-05-05 修回日期:2014-09-17 接受日期:2014-10-14 出版日期:2015-03-10 发布日期:2015-03-10
通讯作者: 张正坤
基金资助:
黑龙江省自然科学基金项目“转MAD1工程基因球孢白僵菌工程菌株防治玉米螟配套技术研究”(C201307)；吉林省科技厅国际科技合作项目“中、俄球孢白僵菌遗传多样性研究与利用”(20110751)。

Research on Effect on Biological Characteristics and Virulence of Beauveria bassianaInduced by Periostracum Cicadae

Guan Bingbing¹, Wang Bing¹, Liu Yanwei¹, Meng Xinrui¹, Li Xiaohui¹, Zhang Jiashi²,
Xu Wenjing², Li Qiyun², Feng Shudan¹, Zhang Zhengkun²

(¹Harbin Normal University, Harbin 150025;
²Institute of Plant Protection, Jilin Academy of Agriculture Sciences, Gongzhuling Jilin 136100)

Received:2014-05-05 Revised:2014-09-17 Accepted:2014-10-14 Online:2015-03-10 Published:2015-03-10

摘要/Abstract

摘要： 利用改良蝉蜕诱导培养基对球孢白僵菌进行诱导，以无蝉蜕PDA培养基作为对照，目的是比较蝉蜕诱导后球孢白僵菌菌株的生长速率、产孢量、孢子萌发率等多个生物学指标，及二者对3龄亚洲玉米螟幼虫的毒力。经蝉蜕诱导的球孢白僵菌菌株的产孢量、孢子萌发率以及对玉米螟幼虫毒力都高于对照组，且差异显著，对玉米螟幼虫毒力处理组LT₅₀为4.73天，对照组需要6.97天。同时经蝉蜕诱导过的菌株开始产孢时间和胞外蛋白酶活性都优于对照组，胞外蛋白酶活性的变化范围是1.497×10^-2~3.538×10^-2 IU/mL，但经诱导菌株的生长速率低于处理组。鉴于球孢白僵菌菌株经蝉蜕诱导后，其产孢量和孢子萌发率增加，并且能够增强对玉米螟幼虫毒力，可利用其进行球孢白僵菌菌种保存和改良。

关键词: 山羊, 山羊

Abstract: Improved medium contained Periostracum Cicadae was used to induce the entomogenous fungus Beauveria bassiana, with the PDA medium as control. The growth rate, sporlation, spores germination and virulence against the third?instar?larva of Ostrinia nubilalis of the fungus were compared on both medium. The results indicated that sporlation, spores germination and bioactivity against pests induced by P. Cicadae were higher than that of the control, respectively, and showed significant difference. LT₅₀ of fungus induced by P. Cicadae against the pests was 4.73 days, and that of the control was 6.97 days. In addition, the sporulation time of the fungus induced and extracellular protease activity by improved medium was better than those of control group, The variation range of extracellular protease activity by improved medium was 1.497×10^-2-3.538×10^-2 IU/mL, but trowth rate of the former was lower than that of the later. In conclusion, the sporlation, spores germination, and virulence against pests were enhanced by P. Cicadae induction, it was potential for storage and improvement of B. bassiana strain.

关兵兵,王冰,刘艳微,孟鑫睿,李晓慧,张佳诗,徐文静,李启云,冯树丹,张正坤. 蝉蜕诱导对球孢白僵菌生物学特性及其毒力影响的研究[J]. 中国农学通报, 2014, 30(34): 51-55.

关兵兵, 王冰, 刘艳微, 孟鑫睿, 李晓慧, 张佳诗, 徐文静, 李启云, 冯树丹 and 张正坤. Research on Effect on Biological Characteristics and Virulence of Beauveria bassianaInduced by Periostracum Cicadae[J]. Chinese Agricultural Science Bulletin, 2014, 30(34): 51-55.

参考文献

[1] 林海萍,韩正敏,张昕,等.球孢白僵菌研究现状及提高其杀虫效果展望[J].浙江林学院学报,2006,23(5):575-580.
[2] Feng M G, Poprawski T J, Khachatorians G G, et al. Production for mulation and application of the entomopathogenic fungus Brauveria bassiana for insect control:current status[J]. Biocontrol Science and Technology,1994,4:3-34.
[3] 蔡守平,何学友,曾丽琼,等.感染星天牛幼虫高致病力金龟子绿僵菌菌株的筛选[J].中国生物防治学报,2012,28(2):293-297.
[4] 冯明光.胞外蛋白酶和酯酶活性作为球孢白僵菌毒力指标的可靠性分析[J].微生物学报,1998,38(6):461-467.
[5] 王清海,万平平,黄玉洁,等.虫生真菌在害虫生物防治中的应用研究[J].山东科学,2005,18(4):37-41.
[6] 曲京峰.蝉蜕[J].山东中医药大学学报,1999,23(1):57-64.
[7] 林华峰,李世广,张磊,等.绿僵菌大孢变种的生物学特征及其对蛴螬的毒力研究[J] .应用生态学报,2006,17(2):351-353.
[8] 王宝辉,郑建伟,黄大庄,等.绿僵菌MS01菌株的生物学特性及在不同温湿度下对光肩星天牛幼虫的致病力[J].林业科学,2009,45(9):158-162.
[9] Charnley A K, St. Leager R J. The role of cuticle-degrading enzymes in fungal pathogenesis in insects[M]. Cole E T. Hoch H C. Fungal spore disease initiation in plants and animals New York, London: Plenum Press,1991:267-287.
[10] Fang W, Leng B, Xiao Y. Cloning of Beauveria bassiana chitinase gene Bbchitl and its application to improve fungal strain virulence[J]. Applied and Environmental Microbiolgy, 2005, 71: 363-370.
[11] 彭国雄,张永军,杨星勇,等.球孢白僵菌不同世代菌株胞外蛋白酶与毒力的关系[J].中国生物防治,1999,16(2):61-64.
[12] Rajanikanth P, Subbaratnam G V, Rahaman S J, et al. Effect of frequency of subculturing of different isolates of Beauveria bassiana Vuillemin on their biological properties[J].Bio-re-source Management,2011,2(1):60-65.
[13] 孙召朋,张正坤,徐文静,等.白僵菌培养基继代培养对蛋白酶(cdep1)、几丁质酶(chit1)基因表达的影响[J].植物保护,2012,38(2):66-70.
[14] 杨星勇,王中康,夏玉先,等.球抱白僵菌凝乳弹性蛋白酶(B b Prl )的纯化与特性[J].菌物系统,2000,19(2):254-260.
[15] 蔡国贵,林庆源,徐耀昌,等.白僵菌菌株退化与培养条件关系及其控制技术[J].福建林学院学报,2001,21(1):76-79.

蝉蜕诱导对球孢白僵菌生物学特性及其毒力影响的研究

Research on Effect on Biological Characteristics and Virulence of Beauveria bassianaInduced by Periostracum Cicadae

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