欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (28): 153-158.doi: 10.11924/j.issn.1000-6850.2014-1417

• • 上一篇    下一篇

乌拉尔甘草毛状根的诱导及离体培养

郭生虎,王敬东,马洪爱   

  1. 宁夏农林科学院,宁夏农林科学院,宁夏农林科学院
  • 收稿日期:2014-05-16 修回日期:2014-05-16 接受日期:2014-07-24 出版日期:2014-10-15 发布日期:2014-10-15
  • 通讯作者: 郭生虎
  • 基金资助:
    宁夏自然科学基金项目“甘草毛状根培养系统建立及新型再生种苗培育研究”(NZ12256)；宁夏农林科学院自主研发项目“甘草毛状根培养 系统建立及次生代谢产物研究”(NKYG-11-04)

Induction and in Vitro Culture of Hairy Roots of Glycyrrhiza uralensis Fisch

  • Received:2014-05-16 Revised:2014-05-16 Accepted:2014-07-24 Online:2014-10-15 Published:2014-10-15

摘要： 为获得甘草的药用成分和保护甘草野生资源，利用发根农杆菌ATCC15834、A4 侵染乌拉尔甘草幼苗、子叶、下胚轴，获得毛状根，建立了乌拉尔甘草毛状根培养系统，经PCR和毛细管凝胶电泳检测，在248 bp 有rolA 基因，在490 bp 有rolC 基因，证实发根农杆菌基因片段在甘草毛状根的基因组中整合并得到表达，其中无菌实生苗毛状根诱导率达79.5%，毛状根分枝较多，生长速度快，在不添加激素的1/2MS液体培养基中生长明显，培养25 天后，鲜重平均增殖达19.13 倍，乌拉尔甘草毛状根培养体系的建立为进一步利用生物反应器规模化培养甘草药用活性成分提供了参考。

关键词: 城镇污泥, 城镇污泥, 生物沥浸, 脱水, 重金属, 养分

Abstract: To obtain medicinal components and protect the wild resource of Glycyrrhiza uralensis, two Agrobacterium rhizogenes ATCC15834 and A4 were used to induce the hairy root from cotyledons, hypocotyls and seedlings of Glycyrrhiza uralensis Fisch. Hairy root was obtained successfully and the system of hairy root induction was established. There were about 248 bp-rolA gene and 490 bp-rolC detected from the hairy-root by PCR and capillary gel electrophoresis which indicated that the gene segments of Agrobacterium rhizogenes had been integrated and expressed in hairy root of Glycyrrhiza uralensis Fisch. The hairy root induction rate was 79.5% by needle stabbing. The hairy roots grew faster and with much more branches in 1/2MS medium. When cultured for 25 days, the average fresh weight growth rate was 19.13 times. Establishment of hairy root culture system of Glycyrrhiza uralensis Fisch provided a foundation for the industrial production of active drug component in bioreactors.