欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (28): 87-92.doi: 10.11924/j.issn.1000-6850.2014-1457

所属专题： 资源与环境； 烟草种植与生产； 农业生态

• • 上一篇    下一篇

湖北省不同生态区烤烟质体色素代谢差异研究

牛志强,杨永霞,刘国顺,郭利,陈毕顺,孙宇翔   

  1. 河南农业大学,河南农业大学烟草生理生化研究基地1414室,河南农业大学,襄阳市烟草公司,湖北省烟草公司神农架林区烟草公司,湖北省烟草公司神农架林区烟草公司
  • 收稿日期:2014-05-20 修回日期:2014-05-20 接受日期:2014-08-26 出版日期:2014-10-15 发布日期:2014-10-15
  • 通讯作者: 牛志强
  • 基金资助:
    湖北省烟草公司科技重点项目(027Y2012-096)；河南省教育厅科学技术研究重点项目(14B180034)

Research on Metabolic Difference of Plastid Pigments of Flue-cured Tobacco from Different Ecological Zones in Hubei Province

  • Received:2014-05-20 Revised:2014-05-20 Accepted:2014-08-26 Online:2014-10-15 Published:2014-10-15

摘要： 为解决襄阳和神农架生态区烤烟质体色素代谢差异问题，以‘云烟87’为材料，分别种植在湖北襄阳（浓香型产区）和神农架（清香型产区）2 个典型生态区。从细胞组织结构、分子机理以及物质代谢3个层面进行系统研究，探究不同生态环境对烤烟质体色素代谢的影响。超微结构分析表明，中部叶发育前期，襄阳‘云烟87’类囊体片层积累较丰富，发育后期降解更充分。基因表达分析显示，中部叶发育前期，叶绿素降解有关的基因在神农架地区表达更活跃，发育中后期，叶绿素合成有关的基因在襄阳地区表达更强。而类胡萝卜素降解基因的表达襄阳均高于神农架，合成基因除叶龄70 天外，神农架表达更活跃。物质代谢分析表明，生长发育过程中襄阳的叶绿素含量高于神农架，而类胡萝卜素含量则低于神农架，后期差异逐渐变小；烤后样襄阳的类胡萝卜素含量低于神农架，降解产物则相反，叶绿素降解产物新植二烯神农架含量更高。这些差异可能是襄阳烤烟呈现浓香型、神农架烤烟表现淡雅香型的重要原因。这为特色烟叶形成机理的解析提供理论依据和研究思路。

关键词: 综述, 综述

Abstract: To identify the effects of ecological environment on leaf plastid pigments of tobacco, the same tobacco variety (Nicotiana tabacum‘Yunyan87’) was chosen to plant in Xiangyang and Shennongjia, two typical different ecological regions of Hubei Province. The ultra structure and the plastid pigment contents were compared as well as the expression of several key genes related to plastid pigment metabolism in ‘Yunyan87’at different development stages. Results showed that the thylakoid lamella of chloroplast were more in Xiangyang than in Shennongjia at the earlier development stages. While at the later stages, the thylakoid lamella was degraded more completely in Xiangyang. The analysis of gene expression displayed that the expression of the genes related to chloroplast degradation was higher in Shennongjia at the earlier leaf development stages. While the expression of the genes related to chloroplast biosynthesis was higher in Xiangyang at the later stages. Meanwhile, the genes related to carotenoid degradation expressed more active in Xiangyang during the whole developing stages. On the contrary, the genes related to carotenoid biosynthesis showed higher expression levels in Xiangyang than in Shennongjia before the 55th day. The analysis of the plastid pigment contents indicated that the contents of chlorophyll of‘Yunyan87’were more in Xiangyang, but the contents of total carotenoids were the opposite. And the difference decreased gradually in the late stages. After being cured, the contents of carotenoids were more in Shennongjia. And the degradation products contents of carotenoids displayed a higher level in Xiangyang. While as for the neophytadiene, the opposite was the case. These results might be the main reasons why the‘Yunyan 87’in Xiangyang and Shennongjia showed different aroma styles and provide the theoretical basis and research ideas on analysis of the formation mechanism of the characteristic tobacco.