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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (6): 45-50.doi: 10.11924/j.issn.1000-6850.2014-2396

所属专题： 生物技术； 小麦

• 农学 农业基础科学 • 上一篇    下一篇

铅铬胁迫对小麦幼苗抗氧化酶活性和丙二醛含量的影响

杨文玲1,2,巩 涛1,2,刘莹莹1,2,王继雯1,2,李冠杰1,2,刘 莉1,2,甄 静1,2,岳丹丹1,2,慕 琦2,陈国参2   

  1. (1河南省微生物工程重点实验室,郑州 450008；2河南省科学院生物研究所有限责任公司,郑州 450008）
  • 收稿日期:2014-09-02 修回日期:2015-02-06 接受日期:2014-09-29 出版日期:2015-03-20 发布日期:2015-03-20
  • 通讯作者: 杨文玲
  • 基金资助:
    2013年河南省科技开放合作项目“重金属铬污染土壤生物修复关键技术研究”(132106000056)。

Effect of Lead and Chromium Stresses on Antioxidant Enzymes Activities and Malondialdehyde Content of Wheat Seedlings

Yang Wenling1,2, Gong Tao1,2, Liu Yingying1,2, Wang Jiwen1,2, Li Guanjie1,2,Liu Li1,2, Zhen Jing1,2, Yue Dandan1,2, Mu Qi2, Chen Guocan2   

  1. (1Key Laboratory of Microbial Engineering of Henan, Zhengzhou 450008;2Henan Academy of Sciences Institute of Biology Co., Ltd, Zhengzhou 450008)
  • Received:2014-09-02 Revised:2015-02-06 Accepted:2014-09-29 Online:2015-03-20 Published:2015-03-20

摘要： 为探讨铅铬胁迫对小麦的毒害机理，以‘豫麦23’为供试材料，用不同浓度的铅铬处理小麦，研究铅铬单一及复合污染胁迫对小麦过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的影响。结果表明，铅铬胁迫对小麦苗的CAT、POD和SOD具有激活效应，铅铬复合胁迫对小麦苗的CAT影响较大，而铅铬单一胁迫下小麦苗的POD和SOD变化较大。铅铬胁迫对小麦苗的MDA具有抑制效应，铅铬复合胁迫对小麦苗的MDA影响较大，铅铬单一胁迫对小麦苗的MDA影响不明显。说明小麦幼苗中抗氧化酶在铅铬胁迫下对小麦幼苗起到了保护作用，但小麦幼苗对铅铬单一及复合污染胁迫的抗性机理是完全不同的；小麦苗对铅铬胁迫的响应不仅与胁迫的重金属种类有关，而且与胁迫的重金属的质量浓度以及胁迫时间有关。

关键词: 实证分析, 实证分析

Abstract: For studying the toxicity mechanism of lead and chromium stresses on wheat, wheat seedlings, ‘Yumai 23’, were used to investigate the effects of different concentrations of lead and chromium stresses on activities of antioxidative enzymes namely catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) and parameters of oxidative stress malondialdehyde (MDA) contents in wheat seedlings. The results indicated that lead and chromium stresses had activation effect on CAT, POD and SOD. Lead and chromium compound stresses caused notable changes on CAT, while the activities of POD and SOD increased obviously under the lead stress or chromium stress. The MDA contents in wheat seedlings had a reducing trend with lead and chromium stress and it decreased notably under lead and chromium compound stresses. But no significant changes were observed for MDA content in the treatment groups of lead stress or chromium stress. The antioxidative enzymes in wheat seedlings played important role under lead and chromium stresses, but it were completely different for the response mechanism to lead and cadmium. The response mechanism of wheat seedlings to heavy metals were influenced by the types of heavy metals as well as the concentration and treatment time of heavy metals.