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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (36): 7-10.doi: 10.11924/j.issn.1000-6850.2014-2544

所属专题： 生物技术； 水稻

• 农学 农业基础科学 • 上一篇    下一篇

SO2毒害环境下水稻叶片超氧化物歧化酶活性与

刘子恒1,唐延林2   

  1. (1甘肃民族师范学院教务处,甘肃合作 747000；2贵州大学理学院,贵阳 550025）
  • 收稿日期:2014-09-18 修回日期:2014-12-23 接受日期:2014-11-06 出版日期:2015-03-20 发布日期:2015-03-20
  • 通讯作者: 刘子恒
  • 基金资助:
    国家自然科学基金项目“基于温度和施氮量的水稻品质光谱监测机理研究”(41061039)；甘肃民族师范学院院长基金项目“甘南藏区高原野生苦菜叶片叶绿素与色彩参数的相关性研究”(12-03)。

Correlation Analysis Between Leaf SOD Activation and Spectral Absorption for Rice Under Sulfur Dioxide Fumigation Condition

Liu Ziheng1, Tang Yanlin2   

  1. (1Office of Academic Affairs, Gansu Normal University for Nationalities, Hezuo Gansu747000;2College of Science, Guizhou University, Guiyang 550025)
  • Received:2014-09-18 Revised:2014-12-23 Accepted:2014-11-06 Online:2015-03-20 Published:2015-03-20

摘要： 为了通过吸收光谱快速测量SO2胁迫下水稻叶片超氧化物歧化酶活性，采用小区试验研究了SO2毒害环境下水稻叶片超氧化物歧化酶活性与吸收光谱变量的相关性。结果表明：在整个生育期，相关曲线存在2个的波峰点，位于520~550 nm和720~750 nm 2个波段之间。计算720~ 750 nm和520~550 nm波段光谱吸收率的平均值A1和A2，通过A1和A2构建比值光谱变量(A1/A2)、相加光谱变量(A1+A2)及对数相加变量(lgA1+lgA2)，利用光谱变量反演水稻叶片超氧化物歧化酶活性。研究发现：在水稻整个生育期，对数相加光谱变量与超氧化物歧化酶活性的相关性显著提高。选取对数相加变量构建水稻叶片超氧化物歧化酶活性的模型。为进一步提高吸收光谱快速测量SO2毒害环境下水稻叶片超氧化物歧化酶活性提出了探索性研究。

关键词: 甘蓝型油菜, 甘蓝型油菜, 质不育三系, 早期关联, 杂交种选育

Abstract: In order to measure the SOD activation of rice leaves by absorption spectra under sulfur dioxide fumigation condition, the correlation between the SOD activation and spectral absorption of rice leaf was studied in an area experiment under sulfur dioxide fumigation condition. Results showed that the correlation curve had two peak points in all the growth stages, located between 520-550 nm and 720-750 nm bands. The average A1 and A2 were calculated by 720-750 nm and 520-550 nm bands. The spectral variables were formed by A1 and A2, and the spectral variables infer SOD activation. The study found that, in the whole rice growing period, the Logarithmic sum of variable correlation spectroscopy and superoxide dismutase activity correlated significantly improved. The model of SOD activation was built by selecting logarithm adding. This article proposes some exploring research for measuring the SOD of rice under sulfur dioxide fumigation condition.