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中国农学通报 ›› 2015, Vol. 31 ›› Issue (12): 200-204.doi: 10.11924/j.issn.1000-6850.casb14100118

所属专题: 园艺

• 生物技术科学 • 上一篇    下一篇

不同剂量中子辐射针叶豌豆的M1代效应研究

徐大鹏1,2,姚泽恩1,2,冯虎元3,尹永智1,2   

  1. (1兰州大学核科学与技术学院,兰州 730000;2兰州大学教育部中子应用技术工程研究中心,兰州 730000;3兰州大学生命科学学院,兰州 730000)
  • 收稿日期:2014-10-28 修回日期:2015-04-14 接受日期:2014-12-24 出版日期:2015-05-06 发布日期:2015-05-06
  • 通讯作者: 徐大鹏
  • 基金资助:
    国家重点基础研究发展计划“青藏高原生态环境变化对工程稳定性的影响”(2012CB026105);兰州大学中央高校基本科研业务费专项资金“中子辐射豌豆种子物理作用机制及遗传规律的研究”(lzujbky-2014-168);甘肃省省青年科技基金计划“豌豆种子快中子辐照半致死剂量的测定与生物学效应的初步研究”(1107RJYA036)。

Effects of Different Dosages of Neutron Radiation on Seed Germination and Seedling Growth of Needle Leaf Pea

Xu Dapeng1,2, Yao Ze’en1,2, Feng Huyuan3, Yin Yongzhi1,2   

  1. (1School of Nuclear Science and Technology, Lanzhou University, Lanzhou 730000;2Engineering Research Center for Neutron Application, Ministry of Education, Lanzhou University, Lanzhou 730000;3School of Life Sciences, Lanzhou University, Lanzhou 730000)
  • Received:2014-10-28 Revised:2015-04-14 Accepted:2014-12-24 Online:2015-05-06 Published:2015-05-06

摘要: 研究不同中子吸收剂量对豌豆产生的M1代生物学效应,为放射生物学研究和中子辐射诱变育种提供重要参考。本研究用252Cf源发射的中子对针叶豌豆(Pisum sativum L. var MZ-1)的干种子进行了辐射,研究了M1代豌豆生长过程中形态和生理方面的生物学效应。结果表明:中子辐射(0.24~4.33 Gy)对M1代豌豆的萌发能力和幼根的生长均有一定的促进作用,低吸收剂量的中子辐射(0.38 Gy)能够显著促进豌豆的萌发和幼根的生长,较高中子吸收剂量(1.66、4.33 Gy)的辐射对M1代豌豆苗期株高的生长有抑制作用,较低中子吸收剂量(0.24、0.38、0.84 Gy)的辐射对M1代豌豆苗期株高的生长有促进作用。微剂量(0.24、0.38 Gy)的中子辐射不会引起M1代豌豆的形态变异,中子吸收剂量在0.84~4.33 Gy间,可以引起M1代豌豆分枝致变。以上结果说明,0.24~4.33 Gy的低吸收剂量的中子辐射豌豆干种子能够使M1代豌豆产生生物学效应,且有比较明显的规律,可以用于辐射育种研究。

关键词: 油菜, 油菜, 播期, 干物质累积, 养分累积

Abstract: The aims were to study biological effect of the different neutron absorbed dosages on M1 generation of pea, and provide important cues for radiation biology and neutron mutation breeding. The dry seeds of Pisum sativum L. var MZ-1 were irradiated by 252Cf fission neutrons, and then biological effects of morphologic and physiologic of M1 generation were studied during the growth of pea. The result showed that, the neutron radiation (0.24-4.33 Gy) had promoting effect on germination and radicle growth of M1 generation of peas, low absorbed dose neutron irradiation (0.38 Gy) could promote the germination and radicle growth significantly. The higher neutron absorbed dose (1.66, 4.33 Gy) had inhibition on the plant height of M1 generation of peas. However, the plant height of M1 generation of peas was promoted by radiation of lower neutron absorbed dose (0.24, 0.38, 0.84 Gy). In addition, the morphologic variation on the M1 generation of pea was not shown by Micro dose of Neutron radiation, 0.84-4.33 Gy neutron absorbed dose could cause mutations in the branching of M1 generation of pea. These results indicated that biological effects of radiation were found on M1 generation of peas after low absorbed dose of 0.24-4.33 Gy irradiated dry seeds of pea, and had obvious rules, could be used in radiation breeding.