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中国农学通报 ›› 2015, Vol. 31 ›› Issue (11): 50-53.doi: 10.11924/j.issn.1000-6850.casb14110113

所属专题: 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

黑曲霉和产朊假丝酵母联合发酵生产单细胞蛋白工艺参数的研究

郝怀志,白滨,董俊,丁文姣   

  1. 甘肃省农业科学院畜草与绿色农业研究所,甘肃省农业科学院农业质量标准与检测技术研究所,甘肃省农业科学院畜草与绿色农业研究所,甘肃省农业科学院农业质量标准与检测技术研究所
  • 收稿日期:2014-11-18 修回日期:2015-02-02 接受日期:2015-02-11 出版日期:2015-05-06 发布日期:2015-05-06
  • 通讯作者: 郝怀志
  • 基金资助:
    “十二五”农村领域国家科技计划课题《西北绿洲农牧循环技术集成与示范》子课题《牛羊玉米秸秆青贮和菌体蛋白饲料高效生产技术研究与促成产品研制》(2012BAD14B10-5)

Study on Production Process Parameters of Single Cell Protein Fermented by Aspergillus niger and Candida utilis

  • Received:2014-11-18 Revised:2015-02-02 Accepted:2015-02-11 Online:2015-05-06 Published:2015-05-06

摘要: 为利用黑曲霉和产朊假丝酵母联合发酵玉米秸秆生产单细胞蛋白,笔者利用经航天诱变筛选的高产纤维素酶活菌株黑曲霉ZM-8和产朊假丝酵母SJ2,采用混菌固态发酵方式,研究产朊假丝酵母SJ2接种量、接种时间和联合发酵时间等因素在玉米秸秆为主要原料的培养基中对发酵产物中单细胞蛋白(SCP)含量的影响。结果表明最优发酵条件为先接入1:30黑曲霉,24h后接种5%的产朊假丝酵母,黑曲霉和产朊假丝酵母联合发酵36h。在最优发酵工艺条件下,发酵产物中SCP的含量达到了25.44%。

关键词: 播期, 播期, 春玉米, 土壤温度

Abstract: To produce single cell protein, corn straw was fermented by Aspergillus niger and Candida utilis. Activity strains Aspergillus niger ZM-8 and Candida utilis SJ2 which had high yield of cellulase were screened by space mutation. Single cell protein was produced by fermentation of corn straw using mixed solid state fermentation. Effects of inoculation quantity and vaccination time of Candida utilis SJ2, combined fermentation time and other factors influencing yield of SCP were studied. The results showed that inoculating Aspergillus niger ZM-8 on the rate of 1:30 first, then inoculating 5% Candida utilis SJ2 after 24 hours, and fermenting for 36 hours was the optimal fermentation condition. And the content of SCP in the product reached 25.44% under the optimal condition.