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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (4): 162-166.doi: 10.11924/j.issn.1000-6850.casb14110138

• 林学 园艺 园林 • 上一篇    下一篇

基于再生不定芽的蝴蝶兰继代扩繁体系建立

彭 娇,崔金腾,王爱香,赵和文   

  1. (北京农学院园林学院,北京 102206）
  • 收稿日期:2014-11-21 修回日期:2014-12-26 接受日期:2015-01-09 出版日期:2015-03-20 发布日期:2015-03-20
  • 通讯作者: 赵和文
  • 基金资助:
    北京市教委科技提升计划项目延庆四季“延庆四季花海景观植物新品种创制与产业化”(PXM2013-014207-000079)、(PXM2014-014207-000081)；北京市属高等学校创新团队建设项目(IDHT20150503)；北京市教委科技创新平台项目“农业综合试验站与教授工作站建设项目”(PXM2014-014207-000018)。

Building of the Phalaenopsis’ Subculture Multiplication System via Regenerating Adventitious Buds

Peng Jiao, Cui Jinteng, Wang Aixiang, Zhao Hewen   

  1. (College of Landscape, Beijing University of Agriculture, Beijing 102206)
  • Received:2014-11-21 Revised:2014-12-26 Accepted:2015-01-09 Online:2015-03-20 Published:2015-03-20

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摘要： 为降低蝴蝶兰通过再生原球茎途径频繁继代培养可能出现的变异及玻璃化风险、减轻继代培养中的褐化，以蝴蝶兰花梗茎段再生的营养芽为试验材料，进行基于再生不定芽的继代扩繁及抗褐化研究。结果显示，以5节花梗的中间腋芽茎段为材料，在VW+ 6-BA 5 mg/L+ NAA 0.1 mg/L+蔗糖30 g/L培养基上进行启动培养，能获得最高的营养芽诱导率。继代培养中，TDZ促进再生不定芽的效果比6-BA强。茎芽经过“切叶”处理，在培养基VW+ TDZ 1 mg/L+300 mg/L柠檬酸+10 g/L蔗糖+15%椰汁，或培养基VW+ 6-BA 5 mg/L+ NAA 0.1 mg/L+ 300 mg/L柠檬酸+10 g/L蔗糖+15%椰汁上进行继代培养，能获得最佳的继代扩繁效果。笔者研究认为初步建立了基于再生不定芽的蝴蝶兰继代扩繁体系。

关键词: 柑橘, 柑橘, SSR, 聚类分析, 亲缘关系

Abstract: To reduce the possibilities of genetic variation or vitrification during continuous subculture via regenerating protocorms and to alleviate the browning, a Phalaenopsis’ subculture technique via regenerating adventitious buds from vegetative buds produced from the flower stalks and applying anti-browning agent was studied. The results showed that: the highest vegetative bud induction rate was achieved when the middle auxiliary bud stem segments from the five-nodes flower stalks were inoculated in the medium of VW 6-BA 5 mg/L NAA 0.1 mg/L sugar 30 g/L. In subculture, TDZ was more effective than 6-BA in stimulating adventitious buds regeneration. The best subculture multiplication efficiency was achieved when the inoculating buds treated with ‘cutting leaf’ were cultured in the medium VW TDZ 1 mg/L citric acid 300 mg/L sugar 10 g/L coconut water 15%, or in the medium VW 6-BA 5 mg/L NAA 0.1 mg/L citric acid 300 mg/L sugar 10 g/L coconut water 15%. Through studies, the Phalaenopsis’ subculture multiplication system was preliminarily set up via regenerating adventitious buds.