欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (31): 285-290.doi: 10.11924/j.issn.1000-6850.casb15030242

所属专题： 生物技术

• 食品 营养 检测 安全 • 上一篇    

植物乳杆菌Zhang-LL 高密度发酵的研究

白永强,于立娟,周慧敏,段慧霞,高鹏飞,刘晓军,刘 慧,张红星   

  1. 北京和美科盛生物技术有限公司,北京农学院食品科学与工程学院,北京农学院食品科学与工程学院,北京农学院食品科学与工程学院,北京和美科盛生物技术有限公司,北京和美科盛生物技术有限公司,北京农学院食品科学与工程学院,北京农学院食品科学与工程学院
  • 收稿日期:2015-03-29 修回日期:2015-07-28 接受日期:2015-08-24 出版日期:2015-11-16 发布日期:2015-11-16
  • 通讯作者: 张红星
  • 基金资助:
    北京市属高等学校高层次人才引进与培养计划“天然乳酸菌素对畜产品中重要致病菌的抑菌机制及作用研究”(CIT&TCD20140315)；“十二五”国家863 科技计划子课题“畜产品病原菌安全控制技术”(2012AA101606-5)。

High Cell Density Culture of Lactobacillus plantarum Zhang-LL

白永强,,,,,, and   

  • Received:2015-03-29 Revised:2015-07-28 Accepted:2015-08-24 Online:2015-11-16 Published:2015-11-16

PDF (PC)

380

摘要： 本试验旨在通过优化培养条件提高植物乳杆菌Zhang-LL发酵培养液中的菌体浓度，为获得高活菌数的益生菌产品奠定基础。对植物乳杆菌的发酵培养基中的氮源进行单因素筛选，选取501 油菜籽蛋白胨作为MRS培养基的替代氮源。基于优化的培养基成分，采用单因素及4 因素3 水平L9(34)正交试验优化发酵条件，确定最优发酵条件为；501 油菜籽蛋白胨含量为3.0%、温度34℃、pH 6.5、接种量7%，发酵罐流加15% Na2CO3，转速为150 r/min；在此优化条件下，发酵液活菌数可达1.07×1010CFU/mL。

关键词: 悬铃木, 悬铃木, 幼苗, 盐胁迫, 生理指标, 叶绿素荧光

Abstract: This experiment aimed to improve the concentration of Lactobacillus plantarum Zhang-LL in the fermentation broth by optimizing culture conditions, which could lay the foundation for obtaining high survival counts of probiotic products. The single factor experiments were carried out to determinate the optimal nitrogen sources of fermentation medium, and rapeseed peptone (501) was the alternative nitrogen source in the MRS culture medium. Then, the single factor experiments and orthogonal experiment L9(34) were employed to optimize fermentation conditions based on the optimized medium. The optimum fermentation conditions were determined as follows: the content of nitrogen source was 3.0%, temperature 34℃, pH 6.5, inoculation 7%, stirring speed 150 r/min, and the pH value controlled at 6.5 by chemical neutralization with 15% Na2CO3. The highest bacterial counts of Lactobacillus plantarum was 1.07×1010 CFU/mL on the optimal conditions.