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中国农学通报 ›› 2015, Vol. 31 ›› Issue (33): 230-235.doi: 10.11924/j.issn.1000-6850.casb15040012

所属专题: 生物技术

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

褐潮土中外源镍对植物根伸长和蚯蚓生殖的毒性效应研究

张露,段碧华,颜增光,苑亚会,白陈仲,徐猛,王敬贤,梁琼   

  1. 北京农学院园林学院,北京农学院园林学院,中国环境科学研究院环境基准与风险评估国家重点实验室,中国环境科学研究院环境基准与风险评估国家重点实验室,中国环境科学研究院环境基准与风险评估国家重点实验室,中国环境科学研究院环境基准与风险评估国家重点实验室,北京农学院植物科学技术学院,北京农学院植物科学技术学院
  • 收稿日期:2015-04-01 修回日期:2015-05-31 接受日期:2015-06-15 出版日期:2015-11-26 发布日期:2015-11-26
  • 通讯作者: 段碧华
  • 基金资助:
    国家自然科学基金“轻度污染土壤中镍的生物可利用性及其刺激蚯蚓生殖的Hormesis 效应研究”(41271492);北京市自然科学基金“草坪根腐病拮抗放线菌几丁质酶基因的克隆与表达”(5152006);北京市教委科研计划项目“设施土壤中残留杀菌剂的光解去除机制及应用”(KM201310020003)。

Toxic Effect of Added Nickel on Plant Root Elongation and Earthworm Reproduction in Fluvo-aquic Soil

  • Received:2015-04-01 Revised:2015-05-31 Accepted:2015-06-15 Online:2015-11-26 Published:2015-11-26

摘要: 土壤污染物基础生态毒理数据是开展污染土壤生态风险评估和构建土壤生态筛选基准的重要依据。以北京褐潮土为试验介质,采用ISO 11269-1—2012 标准方法,在土壤中添加0、10、18、32、56、100、180、320、560、1000、1780 mg/kg 外源镍,测定镍对单子叶植物大麦、小麦、燕麦和双子叶植物黄瓜、生菜、油菜根伸长的影响,并检测土壤中镍的残留含量。同时,以此系列浓度(除1780 mg/kg 以外)的镍污染土壤为测试介质,测定了镍对赤子爱胜蚓(Eisenia fetida)生殖(产茧量)和生长(体重变化)的影响。植物毒性试验结果表明:褐潮土中低剂量的镍可以促进大麦、小麦根系的生长,表现出一定的毒物兴奋效应,而在高浓度下,镍对6 种植物的根伸长均表现出明显的抑制作用,镍抑制大麦、小麦、燕麦、黄瓜、生菜和油菜根伸长的EC50 值分别为918.3、696.6、837.5、409.3、232.3、608.1 mg/kg,镍对双子叶植物的毒性普遍大于对单子叶植物的毒性。蚯蚓毒性试验结果表明:在0~1000 mg/kg 的处理浓度范围内,镍对蚯蚓体重无明显影响,但在浓度大于180 mg/kg 时对蚯蚓生殖表现出较强毒性,可明显降低蚯蚓的产茧量。北京褐潮土中镍影响蚯蚓生殖的EC50 值为329.6 mg/kg,研究结果可为制定镍的土壤生态基准和开展镍污染土壤生态风险评估提供基础参考数据。

关键词: 水稻, 水稻, 剑叶角度, 氮营养效率

Abstract: The basic ecotoxicological data of soil pollutants are essential to the assessment of ecological risks and the development of ecological soil screening levels. Fluvo- aquic soil was used as the test substrate, artificially contaminated soils were made by adding nickel with the concentrations of 0, 10, 18, 32, 56, 100, 180, 320, 560, 1000 and 1780 mg/kg. Nickel toxicity to root elongation of monocotyledons (barley, wheat and oat) and dicotyledons (cucumber, lettuce and rape) were evaluated by the method of ISO 11269-1—2012, and the residue amount of nickel in the soil was determined. Meanwhile, nickel toxicity to reproduction (cocoon production) and the growth (body weight change) of earthworm Eisenia fetida were studied using the series of nickel contaminated soils (except the soil of 1780 mg/kg Ni). The hytotoxicity test results showed that low levels of added nickel could promote the elongation of barley and wheat roots, indicated that there was a hormesis. However, high levels of nickel could significantly inhibit the root elongation of the six plants. The EC50 values of root elongation of barley, wheat, oats, cucumber, lettuce, and rape were 918.3, 696.6, 837.5, 409.3, 232.3 and 608.1 mg/kg, respectively. Nickel toxicity to the root elongation of dicotyledons was generally larger than that of onocotyledons. The earthworm toxicity test results showed that nickel at the range of 0- 1000 mg/kg in fluvo-aquic soils had no significant effect on the body weight of earthworms. However, nickel at the concentration of 180 mg/kg or more showed significant toxic effect on earthworm cocoon production, which lead to a decrease in the roduction of cocoon. The EC50 value of the cocoon production was 329.61 mg/kg. The data obtained in this study could promote the development of ecological soil criteria and the assessment of ecological risk of nickel.