欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (25): 176-181.doi: 10.11924/j.issn.1000-6850.casb15050039

所属专题： 油料作物； 园艺

• 植物保护 农药 • 上一篇    下一篇

硒对油菜菌核病菌的抑制作用

贾 芬,朱 婷,赵小虎,生云鹏,明佳佳,贾玮,刘苑坤   

  1. 华中农业大学资源与环境学院/农业部长江中下游耕地保育重点实验室,华中农业大学资源与环境学院/农业部长江中下游耕地保育重点实验室,华中农业大学资源与环境学院/农业部长江中下游耕地保育重点实验室,华中农业大学资源与环境学院/农业部长江中下游耕地保育重点实验室,华中农业大学资源与环境学院/农业部长江中下游耕地保育重点实验室,华中农业大学资源与环境学院/农业部长江中下游耕地保育重点实验室,华中农业大学资源与环境学院/农业部长江中下游耕地保育重点实验室
  • 收稿日期:2015-05-07 修回日期:2015-07-27 接受日期:2015-06-25 出版日期:2015-09-23 发布日期:2015-09-23
  • 通讯作者: 赵小虎
  • 基金资助:
    国家自然科学基金“硒提高油菜对菌核病抗性的生理及分子机制”(31201501)；湖北省自然科学基金“硒调控油菜菌核病根际土壤微生物区系及其效应研究”(2015CFB582)；中央高校基本科研业务费专项资金“硒通过线粒体活性氧途径增强油菜对核盘菌抗性研究”(2014JC007)；“十二五”国家科技计划“城郊区农产品安全提升共性关键技术研究与示范”(2014BAD14B02)。

Inhibition of Selenium on Rape Sclerotinia sclerotiorum

  • Received:2015-05-07 Revised:2015-07-27 Accepted:2015-06-25 Online:2015-09-23 Published:2015-09-23

PDF (PC)

366

摘要： 为明确离体条件下硒能否抑制油菜菌核病病原菌——核盘菌的生长，采用平板培养和液体培养基培养的方式，研究了不同浓度的外源硒对核盘菌菌核的萌发、生长、抑制或致死效果以及菌丝细胞蛋白质和核酸物质外渗的影响。结果表明：硒对核盘菌的生长表现出显著的抑制作用(P＜0.05)，其抑制中浓度(EC50)为2.23 mg/L；外源硒仅抑制了核盘菌的生长，并未使其致死；低浓度的硒(＜5 mg/L)未显著抑制核盘菌菌核的萌发，当硒的浓度达到50 mg/L时，菌核的萌发抑制率达100%；硒作用下培养基中蛋白质(OD280)和核酸类物质(OD260)的含量增加，且二者的含量均随硒浓度的增加呈现递增的趋势，反映出外源硒显著增加了核盘菌细胞膜的通透性。研究结果证实，适量的外源硒可显著抑制油菜菌核病原菌的生长。研究结果将为促进微量元素硒在农业安全生产等领域的应用提供理论依据。

关键词: 规划, 规划, 现代农业, 产业转型

Abstract: To determine whether Se can inhibit the growth of Sclerotinia sclerotiorum which was isolated from rape or not, experiments of plate culture and liquid medium culture were used here. In the present study, the germination, growth, inhibition, lethal effect, and the leakage of protein and nucleic acid material in cellular of Sclerotinia sclerotiorum which was treated with different concentrations of exogenous Se were determined. As the results showed, the growth of Sclerotinia sclerotiorum was inhibited significantly (P＜0.05), and the EC50 was 2.23 mg/L. Se only inhibited the growth of Sclerotinia sclerotiorum but not led it to death. Low concentration of Se (＜5 mg/L) had no effect on inhibiting the germination of sclerotia of Sclerotinia sclerotiorum. However, the germination of sclerotia was inhibited totally when the concentration of Se was higher than 50 mg/L. The leakage of protein (OD280) and nucleic acid material (OD260) in cellular of Sclerotinia sclerotiorum increased with the treatment of Se, which indicated that the permeability of cell membrane was enlarged significantly with the addition of Se in the media. In conclusion, exogenous Se can inhibit the growth of Sclerotinia sclerotiorum. The results of the present study provide a theoretical basis for the safe agricultural action and the use of the micro-element Se.