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中国农学通报 ›› 2015, Vol. 31 ›› Issue (28): 13-17.doi: 10.11924/j.issn.1000-6850.casb15060054

所属专题: 园艺

• 林学 园艺 园林 • 上一篇    下一篇

桐花树果实乙酸乙酯部位体外抗氧化活性研究

蔡建秀1,顾雅青1,黄晓冬1,2,黄建新3,李元跃2,朱 晓1   

  1. (1泉州师范学院近海资源生物技术福建省高校重点实验室,福建泉州 362000;2福建省海洋渔业资源与生态环境重点实验室,福建集美 361000;3福建医科大学附属第二医院,福建泉州 362000)
  • 收稿日期:2015-06-09 修回日期:2015-07-21 接受日期:2015-07-24 出版日期:2015-10-27 发布日期:2015-10-27
  • 通讯作者: 蔡建秀
  • 基金资助:
    2015年福建省(国家级)大学生创新创业训练计划项目“桐花树果实各部位萃取、体外抗氧化作用和多酚提取、抑制α-糖苷酶活性研究”(201510399003);福建省自然科学基金项目“红树植物桐花树叶片抗炎镇痛有效部位的开发”(2015J01146);福建省海洋渔业资源与生态环境重点实验室开放基金资助项目“红树植物桐花树叶片抗炎镇痛活性部位及其化学成分研究”(fmfre2014011);泉州市科技计划B类项目“桐花树提取物防治骨性关节炎的药效学与机制研究”(Z20140156)。

Antioxidant Activity in vitro of the Ethylacetate Fraction from Aegiceras corniculatum Fruit

Cai Jianxiu1, Gu Yaqing1, Huang Xiaodong1,2, Huang Jianxin3, Li Yuanyue2, Zhu Xiao1   

  1. (1Fujian Advanced Education Key Laboratory of Inshore Resources Biotechnology, Quanzhou Normal University, Quanzhou Fujian 362000;2Fujian Provincial Key Laboratory of Marine Resources and Eco-environment, Jimei Fujian 361000;3The Second Affiliated Hospital of Fujian Medical University, Quanzhou Fujian 362000)
  • Received:2015-06-09 Revised:2015-07-21 Accepted:2015-07-24 Online:2015-10-27 Published:2015-10-27

摘要: 为了研究桐花树果实乙酸乙酯部位的抗氧化作用,考察了乙酸乙酯部位对DPPH自由基,羟自由基的清除效果,测定了其总还原力以及对H2O2诱导小鼠氧化溶血和对小鼠肝匀浆MDA生成的抑制作用。结果表明:桐花树果实乙酸乙酯部位对DPPH自由基和羟自由基的清除率随着样液质量浓度的增加而升高。0.01、0.05、0.1 mg/mL桐花树果实乙酸乙酯部位对H2O2对小鼠红细胞溶血抑制的抑制率分别为47.64%、67.09%、81.99%。与阴性对照组相比,0.5~2.0 mg/mL桐花树果实提取物均可极显著地抑制小鼠肝匀浆自发的产生MDA(P<0.01)。可见,桐花树果实乙酸乙酯部位具有明显的抗氧化活性。

关键词: 油松, 油松, 端粒酶, TRAP法, 优化

Abstract: To study the antioxidation of the ethylacetate fraction from Aegiceras corniculatum fruit, the scavenging effects of ethylacetate fraction on DPPH and hydroxyl radical were investigated in vitro, total reducing power was determined. The inhibiting effects of ethylacetate fraction on mice RBC (the red blood cell) hemolysis induced by H2O2 and on MDA (malondialdehyde) formation of mice liver homogenate were also examined. The results were as follows: the clearance rate of DPPH and hydroxyl radical of the ethylacetate fraction from Aegiceras corniculatum fruit increased with its mass concentration increasing. Under the concentration of 0.01, 0.05, 0.1 mg/mL, the inhibitory rate of ethylacetate fraction on mice RBC hemolysis by H2O2 inducement was 47.64%, 67.09%, 81.99%, respectively, and under the concentration from 0.5 mg/mL to 2.0 mg/mL, the ethylacetate fraction inhibited MDA production of mice liver homogenate very significantly (P< 0.01) compared with negative control group. Thus, it was clear that the ethylacetate fraction from Aegiceras corniculatum fruit had obvious antioxidant activity.