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中国农学通报 ›› 2015, Vol. 31 ›› Issue (29): 59-64.doi: 10.11924/j.issn.1000-6850.casb15070106

• 生物技术科学 • 上一篇    下一篇

高取代度羧甲基茯苓多糖(CMP)的制备及其注射剂的研制

林标声,陈小红,罗茂春   

  1. 龙岩学院生命科学学院,龙岩学院生命科学学院,龙岩学院生命科学学院
  • 收稿日期:2015-07-19 修回日期:2015-08-19 接受日期:2015-08-25 出版日期:2015-10-28 发布日期:2015-10-28
  • 通讯作者: 罗茂春
  • 基金资助:
    龙岩学院产学研合作项目“发酵茯苓多糖保健品、注射剂的研制及其药理作用研究”(LC2013010),龙岩学院“百名青年教师攀登项目”“发酵茯苓多糖的提取、纯化及改性研究”(LQ2013024),

Preparation of carboxymethylpachymaran(CMP) with high degree of substitution and its injection

  • Received:2015-07-19 Revised:2015-08-19 Accepted:2015-08-25 Online:2015-10-28 Published:2015-10-28

摘要: 为研究高取代度羧甲基茯苓多糖(CMP)及其注射剂的制备工艺条件,以中药茯苓粉为原料,正交试验优化其羧甲基反应的工艺条件,并对所制备的多糖注射剂进行性能鉴定和药理学试验。结果表明:所获得的羧甲基茯苓多糖取代度高、纯度高,具有羧甲基反应特征,最优反应工艺条件为氯乙酸:氢氧化钠=1.15:1,反应温度75℃,反应时间3 h,此最优组合下羧甲基取代度(DS)值为1.21。所制备的多糖注射剂性状稳定、无毒、无热原性和无刺激性,安全可靠。羧甲基茯苓多糖具有直接抑制或杀死肿瘤细胞、增强巨噬细胞吞噬、调节机体免疫功能的药理作用。高取代度羧甲基茯苓多糖及其注射剂的研制可为丰富茯苓多糖的产品类型、拓宽应用范围及市场投入提供前期的理论基础。

关键词: 续随子, 续随子, 索氏提取法, 响应曲面法, 脂肪酸, 气相色谱-质谱联用

Abstract: To study the process conditions of carboxymethylpachymaran (CMP) with high degree of substitution and its injection, using Poria cocos powder as raw material, the carboxymethyl reaction process conditions of CMP were optimized by orthogonal experiments. And CMP injection was prepared and tested by performance testing and pharmacology experiment. The results indicated that the obtained CMP had high degree of substitution, high purity and had carboxymethyl reaction characteristics. The optimal process condition for the carboxymethyl group substitution of pachyman was chloroacetic acid: sodium hydroxide=1.15:1, the reaction temperature 75℃, the reaction time 3 h. Under this optimal condition, the degree of carboxymethyl group substitution (DS) was 1.21. The CMP injection was stable, non-toxic, non-pyrogenic and non-irritating, so it was safe and reliable. The CMP could directly inhibit or kill tumor cells, enhance the phagocytosis of macrophages, regulate the immune function of the body. Preparation of CMP and its injection could help to enrich the product type of the polysaccharide, broaden the application scope and provide the early stage theoretical basis for the market investment.

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