交替氧化酶可增强核盘菌对多菌灵的敏感性

doi:10.11924/j.issn.1000-6850.casb15090138

中国农学通报 ›› 2016, Vol. 32 ›› Issue (6): 193-197.doi: 10.11924/j.issn.1000-6850.casb15090138

所属专题：生物技术

交替氧化酶可增强核盘菌对多菌灵的敏感性

梁五生¹,徐婷¹,姚飞¹,李永红²,王政逸¹

(¹浙江大学农业与生物技术学院生物技术研究所/水稻生物学国家重点实验室,杭州 310058；²陕西省杂交油菜研究中心,陕西大荔 715105）

收稿日期:2015-09-30 修回日期:2016-01-29 接受日期:2015-11-24 出版日期:2016-03-07 发布日期:2016-03-07
通讯作者: 梁五生
基金资助:
浙江省教育厅科研计划项目“交替氧化酶在核盘菌对杀菌剂敏感性中的作用”(Y201327327)；浙江省自然科学基金资助项目“油菜转录因子BnWRKY40和BnWRKY75的抗病功能研究”(LY13C140001)；国家自然科学基金资助项目“线粒体和呼吸作用参与化学杂交剂SX-1杀雄的机理研究”(31370279)。

Enhancing Effect of Alternative Oxidase on Sensitivity of Sclerotinia sclerotiorum to Carbendazim

Liang Wusheng¹, Xu Ting¹, Yao Fei¹, Li Yonghong², Wang Zhengyi¹

(¹Institute of Biotechnology/State Key Laboratory of Rice Biology, College of Agriculture and Biotechnology,Zhejiang University, Hangzhou 310058;²Hybrid Rapeseed Research Center of Shaanxi Province, Dali Shaanxi 715105)

Received:2015-09-30 Revised:2016-01-29 Accepted:2015-11-24 Online:2016-03-07 Published:2016-03-07

摘要/Abstract

摘要： 为了探明交替氧化酶是否参与核盘菌对杀菌剂多菌灵敏感性的调控，研究了交替氧化酶对多菌灵抑制核盘菌生长效力的影响，以及多菌灵对核盘菌呼吸活性和交替氧化酶编码基因aox表达的效应。结果发现：抑制交替氧化酶可削弱多菌灵对核盘菌生长的抑制作用，显示交替氧化酶可提高核盘菌对多菌灵的敏感性；多菌灵对核盘菌的细胞色素途径和交替途径活性都没有即时影响；多菌灵预处理24 h对核盘菌aox基因的转录水平没有影响，但对其交替途径活性仍有显著诱导作用。研究结果有助于全面了解多菌灵的杀菌活性调控机制。而且研究结果表明，生产实践中有望应用交替氧化酶的诱导剂或活化剂来提高多菌灵防治核盘菌的效能。

关键词: 梨, 梨, SSR, SRAP, ISSR, 分子遗传连锁图谱

Abstract: In order to study whether alternative oxidase is involved in sensitivity regulation of Sclerotinia sclerotiorum to carbendazim, the influences of this fungicide on mycelial growth, respiration and alternative oxidase encoding gene (aox) expression of S. sclerotiorum were determined. It was found that the inhibition of alternative oxidase alleviated the inhibitory effect of carbendazim on the fungal mycelial growth, indicating that alternative oxidase could increase the sensitivity of S. sclerotiorum to carbendazim. Carbendazim showed no immediate impact on the activities of both cytochrome respiratory pathway and alternative respiratory pathway of S. sclerotiorum mycelia. Pre-treatment for 24 h with carbendazim did not affect the transcript level of aox gene of S. sclerotiorum. However, such pre-treatment obviously induced the alternative respiratory pathway activity of S. sclerotiorum mycelia. The results are helpful for deep understanding of the regulation mechanism of fungicidal activity of carbendazim. Furthermore, they suggest that inducers or activators of alternative oxidase should be applied to acquiring a better controlling effect of carbendazim on S. sclerotiorum in agricultural activities.

中图分类号:

S432.4

梁五生,徐婷,姚飞,李永红,王政逸. 交替氧化酶可增强核盘菌对多菌灵的敏感性[J]. 中国农学通报, 2016, 32(6): 193-197.

Liang Wusheng,Xu Ting,Yao Fei,Li Yonghong and Wang Zhengyi. Enhancing Effect of Alternative Oxidase on Sensitivity of Sclerotinia sclerotiorum to Carbendazim[J]. Chinese Agricultural Science Bulletin, 2016, 32(6): 193-197.

参考文献

[1] Boland G J, Hall R. Index of plant hosts of Sclerotinia sclerotiorum[J]. Can J Plant Pathol,1994,16:93-108.
[2] Bardin S D, Huang H C. Research on biology and control of Sclerotinia diseases in Canada[J]. Can J Plant Pathol,2001,23:88-98.
[3] Young C S, Clarkson J P, Smith J A, et al. Environmental conditions influencing Sclerotinia sclerotiorum infection and disease development in lettuce[J]. Plant Pathol,2004,53:387-397.
[4] Lee Y H, Cho Y S, Lee S W, et al. Chemical and biological controls of balloon flower stem rots caused by Rhizoctonia solani and Sclerotinia sclerotiorum[J]. Plant Pathol J,2012,28:156-163.
[5] 李永红,王灏,李建厂,等.核盘菌对油菜、向日葵和大豆的侵染及其致病性分化研究[J].植物病理学报,2005,35(6):486-492.
[6] Li G Q, Huang H C, Miao H J, et al. Biological control of sclerotinia diseases of rapeseed by aerial applications of the mycoparasite Coniothyrium minitans[J]. Eur J Plant Pathol,2006,114:345-355.
[7] 李伟,李伟,周益军,等.江苏省油菜菌核病菌对多菌灵的敏感性[J].中国油料作物学报,2007,29(1):63-68.
[8] 魏中华,徐娟,郭明霞,等.国内多菌灵的研究进展[J].安徽农业科学,2015,43(3):125-127,141.
[9] 齐永霞,陈方新,苏贤岩,等.安徽省油菜菌核病菌对多菌灵的抗药性监测[J].中国农学通报,2006,22(9):371-373.
[10] Day D A, Whelan J, Millar A H, et al. Regulation of the alternative oxidase in plants and fungi[J]. Aust J Plant Physiol,1995,22:494-509.
[11] 梁五生,梁厚果.抗氰呼吸交替氧化酶研究进展[J].植物学通报,1997,14(2):9-16.
[12] Minagawa N, Koga S, Nakano M, et al. Possible involvement of superoxide anion in the induction of cyanide-resistant respiration in Hansenula anomala[J]. FEBS Lett,1992,302:217-219.
[13] Martins V P, Dinamarco T M, Soriani F M, et al. Involvement of an alternative oxidase in oxidative stress and mycelium-to-yeast differentiation in Paracoccidioides brasiliensis[J]. Eukaryot Cell,2011,10:237-248.
[14] Yukioka H, Inagaki S, Tanaka R, et al. Transcriptional activation of the alternative oxidase gene of the fungus Magnaporthe grisea by a respiratory-inhibiting fungicide and hydrogen peroxide[J]. Biochim Biophys Acta,1998,1442:161-169.
[15] Akhter S, McDade H C, Gorlach J M, et al. Role of alternative oxidase gene in pathogenesis of Cryptococcus neoformans[J]. Infect Immun,2003,71:5794-5802.
[16] Xu T, Yao F, Liang W S, et al. Involvement of alternative oxidase in the regulation of growth, development, and resistance to oxidative stress of Sclerotinia sclerotiorum[J]. J Microbiol,2012,50:594-602.
[17] Xu T, Wang Y T, Liang W S, et al. Involvement of alternative oxidase in the regulation of Sclerotinia sclerotiorum sensitivity to fungicides of azoxystrobin and procymidone[J]. J Microbiol,2013,51:352-358.
[18] Ishii H, Fountaine J, Chung W H, et al. Characterisation of QoI-resistant field isolates of Botrytis cinerea from citrus and strawberry[J]. Pest Manag Sci,2009,65:916-922.
[19] Banno S, Yamashita K, Fukumori F, et al. Characterization of QoI resistance in Botrytis cinerea and identification of two types of mitochondrial cytochrome b gene[J]. Plant Pathol,2009,58:120-129.
[20] Tamura H, Mizutani A, Yukioka H, et al. Effect of the methoxyiminoacetamide fungicide, SSF129, on respiratory activity in Botrytis cinerea[J]. Pesticide Sci,1999,55:681-686.
[21] Affourtit C, Heaney S P, Moore A L. Mitochondrial electron transfer in the wheat pathogenic fungus Septoria tritici: on the role of alternative respiratory enzymes in fungicide resistance[J]. Biochim Biophys Acta,2000,1459:291-298.
[22] Miguez M, Reeve C, Wood P M, et al. Alternative oxidase reduces the sensitivity of Mycosphaerella graminicola to QoI fungicides[J]. Pest Manag Sci,2004,60:3-7.
[23] Yan L, Li M, Cao Y, et al. The alternative oxidase of Candida albicans causes reduced fluconazole susceptibility[J]. J Antimicrob Chemother,2009,64:764-773.
[24] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCt method[J]. Methods,2001,25:402-408.

交替氧化酶可增强核盘菌对多菌灵的敏感性

Enhancing Effect of Alternative Oxidase on Sensitivity of Sclerotinia sclerotiorum to Carbendazim

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	李锐, 尚霄, 尚春树, 常利芳, 闫蕾, 白建荣. SSR荧光检测解析224份山西玉米自交系的遗传结构与遗传关系[J]. 中国农学通报, 2022, 38(5): 9-16.
[2]	武雅娟, 苏本营, 李晶, 高同雨. 基于区块链技术的特色农产品质量供应链设计研究：以“门头沟京白梨”为例[J]. 中国农学通报, 2022, 38(33): 152-157.
[3]	李婷婷, 陈红. 刺梨叶片愈伤组织诱导及抗褐化研究[J]. 中国农学通报, 2022, 38(32): 12-21.
[4]	赵雅儒, 邳植, 刘蕊, 马语嫣, 吴则东. 不同甜菜单胚细胞质雄性不育系与保持系的遗传多样性分析[J]. 中国农学通报, 2022, 38(30): 35-40.
[5]	李佳欢, 王晓慧, 姜明国, 宋福强, 常伟. 基于响应面法优化印度梨形孢培养基[J]. 中国农学通报, 2022, 38(3): 102-109.
[6]	赵明新, 曹刚, 王玮, 曹素芳, 李红旭. 5个梨授粉品种与‘玉露香梨’授粉效应比较研究[J]. 中国农学通报, 2022, 38(28): 52-57.
[7]	马俊杰, 高有华, 阿布力孜, 郭庆元. 库尔勒地区梨木虱发生动态及黄板诱杀效果研究[J]. 中国农学通报, 2022, 38(24): 109-112.
[8]	荣华, 刘龙, 郭庆元. 茶叶活性成分对梨火疫病菌的抑制活性及稳定性研究[J]. 中国农学通报, 2022, 38(24): 118-123.
[9]	钟存, 魏鹏, 张海静. “气候好”农产品贵德软梨评价指标研究[J]. 中国农学通报, 2022, 38(23): 88-94.
[10]	曾少敏, 陈小明, 姜翠翠, 黄新忠. 梨周年无裙膜避雨栽培主要物候期潜在不利气象因子分析[J]. 中国农学通报, 2022, 38(22): 99-103.
[11]	刘龙, 荣华, 郑童童, 马俊杰, 郭庆元. 莫海威芽孢杆菌对梨腐烂病的抑菌防病效果[J]. 中国农学通报, 2022, 38(18): 140-146.
[12]	张佳琦, 郭宗珊, 刘长华, 李荣田. 黑龙江省水稻品种的遗传多样性[J]. 中国农学通报, 2022, 38(17): 1-9.
[13]	梁燕, 韩传明, 周继磊, 孙超, 王翠香, 李春明, 王静, 闵旭峰, 公庆党, 孟晓烨, 杨绪强. 山东核桃良种SSR指纹图谱及分子身份证的构建——基于毛细管电泳分析[J]. 中国农学通报, 2022, 38(15): 113-121.
[14]	张子申, 柯泽华, 赵曙良, 程福厚, 李向玺. 采收期对‘黄冠’梨果实品质影响的研究[J]. 中国农学通报, 2022, 38(1): 39-43.
[15]	王梦柳, 樊卫国, 官纪元, 龚芳芳, 何春丽. 营养液pH变化对刺梨苗吸收硝态氮和铵态氮的影响[J]. 中国农学通报, 2021, 37(9): 28-34.